Effects Of SNMC On Chemokines Of Peripheral Blood Monocytes

Background:SNMC has been widely used in medication of urticaria, eczema,dermatitis,drug eruptions,psoriasis and so on,large amount of clinical researches have proved that SNMC is more effective than antihistamines in treating allergic diseases like urticaria and eczema.SNMC has also been proved to have the both function of anti-inflammation and anti-allergic reaction,such as inhibiting activity of phospholipase A2,strongly inhibiting classic pathway of complement,and blocking metabolism of AA.Most allergic diseases are induced by typeâ… allergy,IgE cross-linking with receptor causes the release of allergic mediator and degranulation of mastocytes,histamine is the main pathologic basis of immediate type allergic reactions;and derivation of arachidonic acid is the main pathologic basis of delayed type allergic reactions. Recent researches raised the definition of allergic inflammation.It is known that allergen can induce mastocytes to synthesize histamine,IL-4,IL-13 and TNF-αand chemokines from Th₂ lymphocytes,fibroblast is activated and produces massive chemokines.In addition to the function of IgE,chemokines and eosinophils also play an important role in the pathogenesis of allergic skin disease.Some new type of anti-histamines such as mizolastine have been proved the effect on other factors of allergic inflammation besides the anti-histamine function,such as 5-lypoxygenas metabolic pathway of arachidonic acid to relieve the skin reaction.Research in vitro has confirmed that mizolastine is capable of deregulating the expression of chemokines from normal skin tissue, thus,we design this protocol to find out whether SNMC has the stronger or similar function or not.Objective:To investigate the influence of SNMC on the RANTES,MCP-1,MIP-1αsecretion and mRNA expression of PBMC,meanwhile,compare with the effects of dexamethasone,loratadine,mizolastine.In order to provide new evidence for treatment of allergic diseases.Methods:PBMC was delivered from peripheral blood of 15 healthy persons, PBMC would be cultivated with dexamethasone,mizolastine,loratadine respectively,and thirty minutes later histamine and arachidonic acid were added. Twenty-four hours later the concentrations of RANTES,MCP-1,MIP-1αin cultivated supernatant were detected by ELISA,the mRNA expression of RANTES,MCP-1,MIP-1αwere analyzed by fluorescence quantitative PCR methods.Results:The result of ELISA is in chart 6,mRNA is in chart 5.From the two charts we can see that the secretion and mRNA expression of MCP-1,RANTES has been increased after cultivating with histamine and AA, and decreased after the medicine added in with different levels.No significant increase has been seen in the secretion of MIP-1 a in supernatant,but significant increase in mRNA expression.Analyzed the samples with fluorescent quantitative PCR instrument,see chart 6.Conclusion:From the mRNA expression and secretion of chemokines, SNMC can inhibit the expression of chemokines mediated by histamine and arachidonic acid approximately as the other antihistamines in this experiment, we found out that the effects of SNMC contains not only anti-histamine but also anti-inflammation,and the effect is similar to mizolastine which its anti-inflammation effect has been confirmed,so as to provide evidence in vitro of SNMC for clinical treatment of allergic diseases including urticaria.