Protective Effects Of DhHP-6 On Intracerebral Hemorrhage And Cerebral Ischemia

Cerebrovascular disease(CVD)is a devastating clinical condition,which severely do harm to human healthy.Both case-fatality rate and Mutilation rate are high.The significance of the peroxidases is in the prevention and therapy of the diseases caused by free radicals, In spite of the therapeutical potential of natural peroxidases,it is severely limited by its source,difficult refinement,instability,molecular mass and other factors;thus enzyme mimetics of peroxidases are desirable. DhHP-6 is enzyme mimetics of peroxidases made by the method of mimic enzym.In our study,to investigate the protective effects of DhHP-6 on intracerebral hemorrhage and ischemic in rats. The firt model of experimental intracerebral hemorrhage was induced in rats through infusion of 50 ul of fresh autologous blood drawn from caudal artery into the caudate uncleus.The changes behavior were observed.Histopathological change of rat brain subjected,Plasma SOD,MDA and NO,as well as brain tissue SOD,MDA and NO levels,were determined.We made intracerebral hemorrhage model in rats to study the effects of DhHP-6 on neurocyte morphology changes and neurological scale. DhHP-6(1.0mg/kg,0.3mg/kg,0.1mg/kg)can reduced neurological scores(P<0.001,P<0.001,P<0.05), NO and MDA generation,decrease SOD activity After intracerebral hemorrhage. DhHP-6 significantly increased SOD content in plasma(P<0.05)and brain tissu(P<0.01,P<0.05)decreased MDA content in plasma(P<0.01, P<0.05)and brain tissue(P<0.01)as well as in plasma(P<0.05)and brain tissue(P<0.05,P<0.05)NO levels.The second model of experimental, Through setting up incomplete cerebral ischemia. the effects of DhHP-6 on the level of SOD,MDA,NO,CK,LDH both in the brain tissue and serum of rats. At the same time,samples were taken to store in the fixation liquid for HE.In our study,DhHP-6(1.0mg/kg)gnificantly increased SOD content in plasma(P<0.01)and brain tissu(P<0.01)decreased MDA content in plasm(P<0.01 ,P<0.01,P<0.05)and brain tissue(P<0.01,P<0.05)as well as in plasma(P<0.05)and brain tissue(P<0.05)CK and LDH levels.DhHP-6 could obviously decrease the content of MDA and could increase the activity of SOD both in brain tissue and serum; .This indicated that DhHP-6 could increase the anti-oxidation activity of the enzyme, enhance the ability of removing free radicals and reduce the free radical from ischemia-reperfusion,so that it improve function of brain. The results of the experiment also demonstrated that DhHP-6 could strickingly reduce the content of NO in brain tissue,which showed that NaoxinKang Injection could ease the harm of NO.The results of the experiment showed that DhHP-6 could significantly reduce the content of CK and LDH, wich indicates that DhHP-6 could relieve the damageand protect the integrity of cellular membrane.