The Detection And Clinical Significance Of PCNA Expression And C-met Gene Abnormality In Gastric Carcinoma

Objective To explore the potential pathogenic role of proliferating cell nuclear antigen(PCNA) and c-met gene abnormality in the oncogenesis of gastric carcinoma.Methods Semi-quantitative PCR was used to detect the amplification of c-met gene in 58 gastric carcinoma tissues and corresponding para-carcinoma tissues. Immunohistochemical staining was used to detect the expression of c-met and PCNA protein.The level of c-met gene amplification was 0.864±0.205 and 0.615±0.194 in gastric carcinoma and correspounding para-carcinoma tissues.Results①The amplification of c-met was detected in 58 cases of gastric carcinoma and corresponding para-carcinoma tissues,the positive rate was 100%. The level of c-met gene amplification was 0.864±0.205 and 0.615±0.194 in gastric carcinoma and correspounding para-carcinoma tissues.There was significant statistic difference on the amplification of c-met oncogene between the two groups(t=9.468, P＜0.01).②The positive rate of c-met protein in gastric carcinoma tissues was 67.24%(39/58),which was negative in the corresponding para-carcinoma tissues. There was significant difference between the two groups(x~2=37.053,P＜0.01).③PCNA labeling index(LI) of 58 gastric carcinoma tissues and the corresponding adjacent tissues were 38.699±10.362 and 22.159±9.532 respectively.The PCNA LI had a significant difference between the two groups(t=8.95,P＜0.01).④The amplification and expression of c-met gene in gastric carcinoma tissues has no relationship with the tumor's size,location,lymph node metastasis,differentiation degree and deeper invasion.⑤PCNA LI of c-met protein positive gastric carcinoma tissues and c-met protein negative gastric carcinoma tissues were 45.321±7.296 and 25.105±5.728,respectively.The PCNA LI had a significant difference between the two groups(t=10.578,P＜0.01).Conclusion①The amplification of c-met oncogene is related with the oncogenesis of gastric carcinoma.②The abnormal expression of c-met and PCNA protein is related with the oncogenesis of gastric carcinoma.The expression of c-met protein might induce the proliferation of cancer cell.③The amplification and expression of c-met gene in gastric carcinoma tissues has no relationship with the tumor's size,location,lymph node metastasis,differentiation degree and deeper invasion.