Hemodynamic Change Of Hepatic Cirrhosis Rat After Orthotopic Liver Transplantation

Aim As a common and frequently-occurring disease, portal hypertension possesses specific regional and systematical hemodynamic characteristic. Final stage benign liver diseases are indication of clinical liver transplantation. Only liver transplantation can cure PHT. There are regional and systematical hemodynamic changes during and after liver transplantation. Study on the hemodynamic changes after liver transplantation in PHT rats can identify the hemodynamic characters before and after liver transplantation. On this foundation, we can investigate the hemodynamic changes in cirrhosis rat after orthotopic liver transplantation. We can investigate the therapic effect and can improve the survival rate of liver transplantation. This study is helpful to enlarge the indication of clinical liver transplantation.Methodâ‘ 100 male SD rats were randomly divided into 5 groups. One group was the control group; The others, as the experiment groups, were investigated after 2,4,7,10 weeks injection with CCl₄ respectively.â‘¡The 1st group included 50 male SD rats, as the recipients, which were duplicated the PHT rats model with injected CCl₄ 10 weeks. The 2nd group included 30 male SD rats, as the donators, which were bought and fed 3 weeks before liver transplantation. The two groups were paired up together in accordance with their weight. The method of two sleeves anastomoses was performed. 3) Electromagnetic blood flow meter was used to detect the portal venous flow. The ileocolic vein, inferior vena cava and abdominal aorta were pricked respectively, and were connect to electrophysiolograph and pressure transducer, so that the value of portal venous pressure, inferior vena cava pressure and mean arterial blood pressure could be determined.Resultsâ‘ During the process of cirrhosis, the hepatocyte underwent four phases including degeneration, necrosis, fibrosis and pseudolobule proliferation. The value of mean arterial pressure, portal venous pressure,portal venous flow, portal vascular resistance and splanchnic vascular resistance in the rats with carbon tetrachloride injecting 2 weeks were different to it in the control group rats(P<0.05). But injection for 2 and 4 weeks, there were no significant deviation. After injection for 4 and 7 weeks, there were significant deviation in mean arterial blood pressure, portal venous pressure, portal venous flow and portal vascular resistance(P<0.05). But after injection for 7 and 10 weeks, there were no significant deviation, except portal vascular resistance(P<0.05).â‘¡After liver transplantation had completed, the value of mean arterial blood pressure and portal venous flow of recipient rose(P<0.05), but the value of portal venous pressure and portal vascular resistance decreased(P<0.05). Conclusions During the duplication of cirrhosis rat model, the portal vascular resistance plays an important role in the forming of portal hypertension. It also affected the hemodynamic changes. The vasoactive substance is important to the portal hypertension forming. The values of portal venous pressure, portal vascular resistance and portal vein flow are decreased after liver transplantation in short term, but the long term effect still need to be studied.