Expression Of VEGF, Flt-1, Flk-1, PCNA And CD34 In Juvenile Nasopharygeal Angiofibroma

Objective To investigate the nature of juvenile nasopharygeal angiofibroma(JNA) through compared the differences of vascular endothelial growth factor(VEGF), VEGF receptor-1/ fms-like tyrosine kinase-1(VEGFR-l/Flt-1),VEGF receptor-2/ fetal liver kinase-1(VEGFR-2/Flk-l),proliferating cell nuclear antigen(PCNA) and CD34's expression between hemangioma and vascular malformation.Methods 28 cases of JNA,20 cases of orbital cavernous hemangioma(all of these were sorted by microscope as vascular malformation) and 10 cases of chronic rhinosinusitis were studied respectively by immunohistochemical S-P methods,to detected the expression of VEGF,Flt-1,Flk-1,PCNA and CD34,and the correlation between the expression rates of VEGF,Flt-1 and Flk-1 was also analyzed.Results The positive rates of VEGF,Flt-1,Flk-1 expression in the endothelial cells of JNA were 96.4%(27/28),100%(28/28),100%(28/28),higher than those in orbital cavernous hemangioma which were 10%(2/20),40%(8/20),45%(9/20), there were significant difference(P＜0.05).The average microvessel density(MVD) marked by CD34 in JNA was(49.3±9.1)/HPF(high power field),higher than orbital cavernous hemangioma which was(29.1±6.7)/HPF,there were significant difference (P＜0.05).The PCNA expression in the endothelial cells of JNA and orbital cavernous hemangioma were both positive,there were no significant difference (P＞0.05).The PCNA expression in stromal cells of JNA were positive(100%),the PCNA expression in stromal cells of orbital cavernous hemangioma were negative, there were extraordinary signifycant difference(P＜0.05).Conclusion The expression of VEGF,Flt-1 and Flk-1 in JNA were higher than those in orbital cavernous hemangioma,and there was a correlation between the expression of VEGF,Flt-1 and Flk-1 in JNA.This showed us the expression of VEGF, Flt-1,Flk-1 may be the important factors and play a crucial role in the development of JNA,the nature of JNA could be a hemangioma.The expression of VEGF,Flt-1 and Flk-1 could be an effective sign to differentiate hemangioma and vascular malformation.