Expression And Biological Significance Of P15, P16, COX-2, PCNA, CD44v6 Protein In Different Procancerous Lesion And Esophageal Squamous Cell Carcinoma Tissue

Objective:To examine the expression of P15,P16,COX-2,PCNA,CD44v6 protein in different procancerous lesion and esophageal squamous cell carcinoma tissue and to investigate the relationship between their expression and the biological level,lymph node metastasis.Methods:Immunohistochemistry was demonstrated to show these tumor markers' expression in different procancerous lesions and esophageal squamous cell carcinoma tissue.Results:Stepwise over-expression of COX-2,CD44v6,PCNA and reduction of P 15,P 16 protein were observed in the muti-step process of esophageal tissue.Significant difference in expression of these tumor markers(P＜0.001)was observed between normal esophageal epithelia and dysplasia.The expression of p16(P＜0.001)had statistical different in biological level but no statistical different(P＞0.05)in lymph node metastasis.The expression of COX-2(P＜0.001)had statistical different in biological level and lymph node metastasis.The expression of CD44v6(P＜0.001)had statistical different in lymph node metastasis.The relationship between COX-2 and CD44v6 was statistical signficant in esophageal squamous cell carcinoma tissue(P＜0.001).Conclusion:Stepwise over-expression of COX-2,CD44v6,PCNA and reduction of P 15,P 16 protein had relation with esophageal squamous cell carcinoma.The reducing-expression of P16 and the overexpression of COX-2and CD44v6 have effect on prognosis of esophageal squamous cell carcinoma.The expression of COX-2 had relationship with that of CD44v6 in esophageal squamous cell carcinoma.