Effects Of Mifepristone On Cytokines Expression Of Endometrial Lesion In A Rat Model

[Objective] To evaluate the effect of mifepristone on EMs rat model on cytokines level of EMs lesion and serum.The correlated cytokines include adhesion (CD44v6,ICAM-1),aggression (MMP2/TIMP-1) and angiogenesis (VEGF/ENS,TNF-α)[Methods] To erect the EMs rat model according to the method of induced EMs animal model. To select the female SD rats (mean weight 250~300g) and a intramuscular injection of estradiol benzoate (0.1ml/kg) to make the animals in estruation at the same time. The left uterus was resected and cut open, then endometrial tissue were sutured on both sides of the peritoneum. The rats were divided randomly into two groups (ten per group): test group was fed with mifepristone,the control group was done nothing; The correlated cytokines of aggression (MMP2/TIMP-1), adhesion (CD44v6,ICAM-1) and angiogenesis (VEGF/ENS,TNF-α) in EMs lesion and serum were detected.[Results] Compared with control group, the test group(mifepristone treated)the cytokine level correlating with aggression, adhesion and angiogenesis decreased significantly: Immunohistochemistry ectopic foci protein expression: experimental group (using mifepristone) MMP-2 expression significantly weaker than the control group, the difference has statistical significance P<0.05; TIMP-1 expression was no significant difference. VEGF expression in the experimental group than the control group, TNF-αexpression than the control group, the differences were statistically significant,P<0.05. ENS expression with the control group, no difference. Real-time PCR detection of mRNA expression of ectopic foci: adhesion indicators: the experimental group CD44v6, ICAM-1 levels were reduced, the difference has statistical significance,P<0.05. Attack indicators: the experimental group MMP-2, TIMP-1 levels were reduced, there is statistical difference , P < 0.05. Change MMP-2/TIMP-1 was no significant difference. Angiogenesis indicators: the experimental group VEGF, TNF-αcontent, there is statistical significance,P<0.05. ENS / ES was no significant difference in content. ELISA detection of serum protein content: adhesion indicators: the experimental group were lower than the control group, CD44, there is statistical significance,P<0.05, the adhesion ability. Experimental group and control group ICAM-1 serum concentration was no significant difference. Attack indicators: serum MMP-2, TIMP-1 and MMP-2 / TIMP-1, the experimental group and control group, no significant difference. Angiogenesis indicators: serum ENS content, the experimental group than the control group, the difference has statistical significance,P<0.05.[Conclusion] Mifepristone can inhibit CD44v6,MMP-2,VEGF,TNF-αmRNA expression in EMs rat model of EMs lesion and serum,reduced the capacity of EMs lesion in adhesion, invasion and angiogenesis.