Anticancer Activities Of The Extracts From Chrysanthemum Morifolium Ramat In Vitro

Purposes:To investigate the anticancer activities of the different extracts from Chrysanthemum Morifolium Ramat(CMREs)in vitro.Methods:(1)The proliferative inhibitory rate of the AGS cell and K562 cell treated with different CMREs at variant concentrations was measured by MTS assay;(2)The cell cycle and apoptosis of the AGS cell treated with different CMREs was analyzed by flow cytemetry.(3)The influence on the ability of colony-forming of AGS cell treated with different CMREs was measured by the limited deliquation cell colony-forming assay on the plate and the cell colony-forming assay in the soft agar.Results:(1)Both H₂O-extract and acetoacetate-extract of the Chrysanthemum Morifolium Ramat(CMR)had the inhibitory effects on the AGS cell and the K562 cell,however,the H₂O-extract showed higher activity than acetoacetate-extracts(P＜0.01). (2)The effects of anti-AGS cell were studied and compared among the three different samples(Samplel,Sample2,Sample3),which were extracted from the H₂O-extract of the CMR according to the different polar fractions:1)On the premise of the same concentrations of the total flavone and the same times that the samples were diluted by(the concentration of the total flavone of each primary samples was 11.5 mg/ml),it was observed that the proliferative inhibitory rates of the AGS cells were different,which were treated respectively by the three samples mentioned above,especially on the concentrations of being diluted by 64 times and 128 times.But the disparity of the cell proliferative inhibitory rates among the three samples had no statistical significance(P＞0.05).2)On the premise of the same concentrations of the total flavone and the same times that the samples were diluted by(the concentration of the total flavone of each primary samples was 11.5 mg/ml),Samplel and Sample2 showed the proliferative inhibitory effect on the AGS cells more effectively than the extract of the flavone of the CMR(FECMR)(P＜0.01).3)Through the limited deliquation cell colony-forming assay on the plate and the cell colony-forming assay in the soft agar,both Samplel and Sample2 had the effect on inhibiting the colony-forming of AGS cells,while both were diluted by 40 times,80 times,160 times,200 times,400 times,800 times.And Sample2 showed higher activity than Samplel on the plate when diluted by 400 times in the soft agar at the concentrations of being diluted by 40 times and 80 times(P＜0.05).4)The flow cytometry showed that Samplel blocked the cells in G₀/G₁-phase when diluted by 40 times,80 times,160 times(P＜0.05)and Sample2 blocked the cells in G₂/M-phase when diluted by 40 times(P＜0.01);at the same time,the quantity of AGS cells of apoptosis increased when the AGS cells were treated by the Samplel diluted by 80 times and by the Sample2 diluted by 40 times(P＜0.05). Conclusions:(1)The anticancer effect was different when AGS cell was treated with different CMREs,the H₂O-extract was better than the acetoacetate-extract,Samplel and Sample2 showed greater effect than the FECMR,and the Sample2 had higher activity than the Samplel.(2)The different CMREs worked through different mechanism,Samplel was probable a G₀/G₁-phase blocker and Sample2 was probable a G₂/M-phase blocker,and both samples induced the apoptosis of AGS cell.