The Expression Of COX-2, BFGF And The Measurement Of Microvessel Density In Prostatic Carcinoma

Objectives: To investigate the expression of COX-2 and bFGF ,the measure of microvessel density ,and their dependabilities and its clinical significance in prostatic carcinoma;to approach the contribution of angiogenesis of COX-2 and bFGF during the course of genesis and development of prostatic carcinoma.Methods: COX-2 and bFGF protein expression and microvessel density in 35 normal prostate(NP),76 benign prostate hyperplasia(BPH) and 58 prostae carcinoma(Pca) was examined with immunohistochemistry .Results: (1) In NP group,the total positive rate of COX- 2 expression was 0%, in BPH group, it was 9.21%,while it expressed in 72.41% of the prostate carcinoma which was significantly higher than the former two groups(p<0.01).The expression of bFGF was on the rise from NP to BPH and Pca(p<0.01), which was 0%, 63.04% and 81.03% respectively.(2) In Pca group, the expression of COX-2 was 72.41%, which was closely related to the grade and clinical stage of prostate carcinoma.The higher the pathological grade and clinical stage of prostatic carcinoma were the higher expression of COX-2(p<0.01).The differences between highly,moderately differentiated and poorly differentiated cancer were significant(p<0.0125).The expression of bFGF was 81.03% , which was relevant to the clinical stage of prostatic carcinoma(p<0.05)but not to the grade (p>0.05).The expression of COX-2 and bFGF was positively correlated in prostate carcinoma(r=0. 536, p<0.01).(3)In NP group the average MVD was 17.02±3.71, and BPH group 28.06±2.32 ,Pca group58.80±15.54.The MVD averages there were significant differences between NP group and BPH group(p<0.01),and the MVD averages of both NP group and BPH group there were significant differences with PCa group(p<0.01).The microvessel enumerations in prostatic carcinoma were increasing followed by the pathological grade and clinical stage of prostatic carcinoma increasing,that was positive related with COX-2 expression(r=0.697, p<0.01) and positive related with bFGF expression(r=0.705, p<0.01).Conclusion: (1) The expression of COX-2 is closely related to the initiation and proliferation of prostate carcinoma. The detection of COX-2 may contribute to the pathological grade,clinical stage and important clinical prognostic judgement of prostatic carcinoma.(2) The expression of bFGF is closely related to the clinical staging of prostate carcinoma, which suggests that high bFGF expression could promote the development of prostate carcinoma.(3)The expression of COX-2 is positively related with the expression of bFGF in prostatic carcinoma,which develops MVD , and promote angiogenesis and development in prostatic carcinoma.