| Objective: Lung cancer is responsible for the cancer deaths in both men and women throughout the world, and is the number-one cause of cancer deaths in China due to incidence and mortality (more than 20%) increasing dramatically over the last 2 decades. And it is estimated that 0.5% new cases will be diagnosed per year and lung cancer remains among the most common types of cancers worldwide in the 21st century. Non-small cell lung carcinoma (NSCLC) is the most common lung cancer, accounting for about 80% of all lung cancers. Epidemiology data shows lung cancer is the most common cause of cancer death in China, since being diagnosed, most of the cases are in advanced stages and 90% died from carcinoma infiltration and metastasis. The occurrence mechanism of lung cancer depends on alteration of multi-factors, multi-stages and multi-genes, involving many kinds of oncogene activation and anti-oncogene inactivation. The incidence, development and subside of lung cancer is strongly correlated with apoptosis damage of malignant carcinomas, reported by recently research. Clusterin is a new apoptosis inhibitor protein and an important factor causing carcinoma, expressing in all kind of tissue and body fluid, attending physiological and pathological progresses, including immune regulation, cell adherion, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions and adjustment of apoptosis and cell cycle. The activation of aspartate-specific cysteine proteases (Caspases) is the most important stage in the complicated apoptosis process, and Caspase-3 is the most important effective number, which responsible for hydrolyses and splitting of all/part key proleases during apoptosis. Once Caspase-3 is activated, the cell apoptosis goes to inreversible stage. This research is to evaluate the expression and clinical significance of Clusterin and Caspase-3, with detecting their expression in NSCLC compared with benign tissue by immunohistochemistry S-P method combining with clinical pathology.Materials and Methods: 52 surgically resected non-small cell lung carcinoma tissues were collected. Among them male 37, female 15;21 cases were squamous cancer, 31 cases were adenocarcinoma;There were well-differentiated 11, moderate 22, poorly-differentiated 19; and there were 28 cases in stage I-II ,and 24 cases in stageâ…¢;and there were lymph nodes metastasis in 27, and no lymph nodes metastasis in 25; 20 benign lung tissues specimens were collected as control groups. All the tissues were fixed in 10% neutral formalin and embedded in paraffin. S-P immmno- histochemical method was performed to detect the expressions of Clusterin and Caspase-3. The data were analyzed by software SPSS 13.0.Results:1.The positive rate of Clusterin in NSCLC (78.85%) ,was significantly higher than that of Clusterin in benign tissues (10%)( P<0.05); There was significant difference of Clusterin expression between pathological grade, and TNM staging, and lymph node metastasis P<0.05; The expression of Clusterin has no significant difference with histological type (P >0.05).2.The positive rate of Caspase-3 in benign tissues (80%) ,was significantly higher than that of Caspase-3 in NSCLC (44.23%)( P<0.05); There was significant difference of Caspase-3 expression between pathological grade,and lymph node metastasis P<0.05; The expression of Caspase-3 has no significant difference with histological type, TNM staging (P >0.05).Conclusions:1.Overexpression of Clusterin was related with pathologied grades and TNM staging and lymph nodes metastasis in NSCLC. Clusterin may participate in the occurrence and development of NSCLC.2.The low expression of Caspase-3 in NSCLC was closely related with pathological grades and lymph node metastasis, and may participate in the occurrence and development of NSCLC.
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