Hyperbaric Oxygen To Cerebral Hemorrhage Model SD Rat Brain In Vasculogenesis And Angiogenesisi Influence

Objective To observe hyperbaric oxygen to cerebral hemorrhage model SD rats behavior study change influence,To research hyperbaric oxygen to cerebral hemorrhage rats brain in blood vessel newborn influence mechanism, Further elaboration hyperbaric oxygen treats cerebral hemorrhage's pathophysiology mechanism.Method The experiment adopt SD rat 120 in total, And divides into stochastically 120 SD rat the blank comparison (40) and the model group (80), The model group manufactures cerebral hemorrhage animal model, Then divides into the control group and the experimental group stochastically, Each group of 40,The hyperbaric oxygen value of pressure hypothesis is 0.20Mpa, Each time the treatment time is 60 minutes, Daily 1 time,Elsewhere after various components the principle completes, After the surgery 4d, 7d, 14d, 21d, 28d draw an item at random each group of rats 8, Carries on the animal behavior study observation and grades,And draw an item at random five, Beheads takes the brain to use around the immunity histochemistry staining method examination cerebral hemorrhage haematoma the VEGF, HIF-1 expression situation.Results The blank comparison group , Contrast a group and the experimental group to Compare: The blank comparison no significant hematoma, The control group and the experimental group compare its P <0.05value to be even after 4d, 7d, 14d ,Has statistics significance. Afterward the control group and experimental group's dropsy absorbs gradually. After the technique 21d, the 28d brain water content compares its P value >0.05. The non-statistics significance. Before the behavior study observation grading result is the control group and experimental group 48h, grades gradually drops by the respective maximum. After The control group and the experimental group present the varying degree in the first four time section the behavior study exceptionally. And after the technique 4d, the 7d control group and the experimental group three grading sum total (langa grading law +Berderson grading law + balance beam walks experiment) to compare, its P value >0.05, The non-statistics significance, After the technique 14d, the 21d control group and the experimental group three grading sum total compares, its P value>0.05, Has statistics significance. After the technique the 28d control group, the experimental group grades restores the normal level. The blank comparison group each time section not obvious haematoma, Obviously HIF-1αand VEGF are extremely few around injection expresses, Around cerebral hemorrhage haematoma stove obviously HIF-1αand VEGF expression, Is higher than the control group obviously in the same time section experimental group the expression. Control group HIF-1αwhen 4d expresses the peak for it, Afterward each time section assumes the declining trend, The experimental group HIF-1αexpression in 4d, 7d, 14d, the 21d viewing time section assumes increases gradually the tendency, 28d viewing time section has the drop. The control group and the experimental group VEGF expression assumes increases gradually the tendency, Control group VEGF is it expresses the peak in 14d, 21d, 28d viewing time section has the drop, Experimental group VEGF is it expresses the peak in 21d, 28d viewing time section has the drop. The experimental group each time section HIF-1αexpression and VEGF the expression assumes related.Conclusion 1 .The hyperbaric oxygen can reduce the hydro-cephalus.2. The hyperbaric oxygen can promote the rats capacity restoration.3. Cerebral hemorrhage rats haematoma area HIF-1αand VEGF expression enhancement.4. The hyperbaric oxygen can promote in obviously the cerebral hemorrhage rats HIF-1αand the VEGF expression promotes the capillaries vaschlogenesis and angiogenesis. Thus promotion hydro-cephalus absorption, Promotes the rat capacity restoration.