The Effect Of Bu Yang Huan Wu Decoction Wtich Stimulated Basid Fibroblast Grouth Factor In Cerebral Ischemia

OBJECTIVE:The cerebral ischemia is the common disease of nerve system.The incidence-rate in acute cerebral vascular diseases is 70～80%,it has higher death and disability rate.To find effective medicine and therapeutic measures to advance the functional restoration of central nervous system after stroke has always been the emphases of the studies in the interrelated subjects,bFGF(basic fibroblast growth factor)is an important neuron nutrition factor and distributes extensively among ripe and unripe central nervous system,bFGF can promote neuron survival and antagonice excitatory amino acid toxicity in vitro.bFGF can go through blood brain barria(BBB),reduce infarction volume and suppress neuronal apoptosis after cerebral ischemia reperfusion.Bu Yang Huan Wu Decoction(BYHWD) is the classic prescription which protected ischemia brain injured and decreaaased mortality and disability-rate.It investigated the effect of BYHWD on the cerebral ischemia through its intervention to new vessels,bFGF and its receptor of ischemia zone. METHODS:①Eithy Sprague-Dawley rats(weight 280-300) were randomly allocated to four groups:sham-operated group,model group,traditional BYHWD group and ultra-fine powder BYHWD.There were 20 rats in every group.The cerebral ischemia rat model was made by occluding the middle cerebral artery(MCAO) with thread.The traditional Decoction and ultra-fine powder Decoction groups were ingested with the liquid medicine of BYHWD after 2 hours of the operation (2ml/rat/day,according to the surface area,it was three times of the dosage of 70kg adult).All groups were executed on the 1d,3d,5d and7d after the operation and once five rats.At the same time there were 5 rats as the normal control group.②The distribution and expression difference of bFGF in the ischemia train tissues of rats in different groups have been investigated with immuohistochemistry technique.③The content of bFGF was detected with ELISA.RESULTS:①Expression of bFGF at Peri-infarct after ischemia increased obviously,but had only a less expression at central-infarct.There was no expression at ischemic contralateral side.Expression of bFGF immune positive cell increased at 1 day,peaks at 3 days,and returns to baseline at 7 days.②The positive cells of bFGF in tradition Decoction and ultra-fine powder Decoction groups were obviously more than those in the modle group on 3d,5d,7d.(p<0.05) ③The regularity of expression of bFGF in traditional Decoction and ultra-fine powder Decoction groups were the same with that in the modle group.And except for 1d,it was higher at all the time pionts.Furthermore,Compared with modle group,it was signinficantly different on 3d,5d,7d.(p<0.05)④The positive cells and regularity of expression of bFGF in ultra-fine powder Decoction groups were more than those in tradition Decoction on 5d,7d.(p<0.05)CONCLUSIONS:①After stroke,the bFGF positive cells and protein extent was raised.②BYHWD could endure the higher level of bFGF after cerebral ischemic. Maybe it was one of the mechanism of BYHWD to defeat the injury in brain.③The effect of ultra-fine powder Decoction was better than tradition Decoction.