Expression Of Snail,E-cadherin And N-cadherin In Gastric Adenocarcinoma

Objective: Gastric carcinoma (GC) is one of the most common malignant tumors in the world that severely threatens people's health, and its mechanism is still unknown,the invasion and metastasis of GC is an important cause of death. Researches show that the EMT is responsible for the invasion and metastasis of tumor,and the Snail protein and the adhesion proteins E-cadherin,N-cadherin in the occurrence of the EMT have played an important role ,so we can infer that Snail,E-cadherin and N-cadherin played an important role in the invasion and metastasis of GC.We investigate the expression of Snail,E- cadherin and N-cadherin protein in gastric adenocarcinoma and their significance to explore their roles in carcinogenesis, invasion and metastasis of gastric adenocarcinoma and to evaluate their clinicopathologic significances.Method: The expression of Snail,E-cadherin and N-cadherin proteins in 112 cases of gastric adenocarcinoma and 79 cases of adjacent tissues were detected by immunohistochemical S-P method, and the correlation with clinicopathological features was analyzed. We useχ~2 test and Spearman rank correlation analysis method to analysis with the help of SPSS 17.0.Results: (1) The positive rate of Snail immunostaining in gastric adenocarcinoma (82.1%)was higher than that in adjacent tissues(35.4%)(P<0.05);The expression of Snail was significantly related with the differential degree,depth of tumour invasion, lymph node metastasis and distant metastasis (P<0.05), but they did not correlate with sex, age, tumour size and clinical stage(P>0.05).(2) The positive rate of E-cadherin immunostaining in gastric carcinoma(33.9%) was lower than that in adjacent tissues(100%)(P<0.05); The expression of E-cadherin was significantly related with the differential degree,histologic type, depth of tumour invasion,lymph node metastasis,distant metastasis and clinical stage(P<0.05), but they did not correlate with sex, age, tumour size(P>0.05).(3) The positive rate of N-cadherin immunostaining in gastric adenocarcinoma (83.3%)was higher than that in adjacent tissues(41.25%)(P<0.05);The expression of N-cadherin was significantly related with the differential degree,depth of tumour invasion, lymph node metastasis and distant metastasis (P<0.05), but they did not correlate with sex, age, tumour size and clinical stage(P>0.05).(4) A negative correlation was found between the expression of Snail protein and E-cadherin protein(r=-0.212, P<0.05). A negative correlation was found between the expression of N-cadherin protein and E-cadherin protein(r=-0.243, P<0.05).Conclusions: The increased expression of Snail,the decreased expression of E-cadherin and the abnormal expression of N-cadherin may play an important role in the development of gastric adenocarcinoma, and they are involved in the processes of invasion and metastasis of gastric adenocarcinoma. The combined detection of E-cadherin,N-cadherin and Snail has positive effects on predicting the invasion and metastasis in gastric adenocarcinoma.