Protective Effect Of Danxing Tongluo Decoction On Cerebral Ischemia Reperfusion Injury In Rats

Objective:Ischemic cerebral vascular disease (ICVD) is one of the common disease with morbidity and mortality rate. After acute cerebral ischemia, the expression of VEGF and CD34 increases obviously, ace- lerated the vascularization of flap,established lateral branches circu- lation,improved brain ischemic injury as the ischemia continued. After cerebral ischemic reperfusion,microvascular proliferation in the borders of the infarction is directly related to the improvement of blood circulation of cerebral ischemia and it might contribute to the restoration and regeneration of injured neurons.According to several years clinical experiences,Professor LiuYun has formed a basic experienced prescription-XingDanTongLuoTang.The pre- vions section has shown that it could significantly improve symptom of Ischemic Stroke. However,the exact mechanism also remains unknown. In this paper,we explore the expression of VEGF and CD34 in rat brain with injury of Cerebral Ischemia and Reperfusion in order to provide evidence for clinical work.Methods:48 healthy SD rats which ignore gender were assigned randomly to 6 groups, 8 rats per group:blank group , ischemia/reperfusion (I/R) group, DXTLD low dosage group , DXTLD midst dosage group, DXTLD high dosage group and Nimodipine(NIMO) group. DXTLD groups were given to DXTLD at dose of 0.75ml/100g,1.15ml/100g,1.5ml/100g;NIMO group at 2ml/100g,once a day. Saline was given to I/R group at 1.0ml/100g weight.and black group was nothing.After 7-days,the rats of all the groups except black group were made into cerebral ischemia reperfusion injury models by thread embolism method. All the rats were killed and their brains were taken out after 2h ischemia and 24h reperfusion injury. The VEGF expression and CD34 expression in brain tissue were measured with immune- ohistochemical method .Results:1.The ultrathin sections of brain tissue is made research about histomorphology by the common HE tacho-staining technique after rat cerebral ischemia and reperfusion:The group of black group has normal cellular structure;The margin of focus of infection comparatively is distinct and the degree of cellular necrosis is more serious in the group of I/R group. The cells are obviously cutaneous dropsy. At the center of ischemic area we can find cellula nervosa necrosis, circum-cell diastem become broader, nucleus shrinkages,caryotheca fragments,chromato-spherite disappears,etc; While in the group of DXTLD high dosage group and NIMO group we can get the outcome that neuronal degeneration fairly light at ischemic region.Besides,cells comparatively regular and full,and there are integrate living cells at the around of semidarkness area.2.To observe:the expression level of VEGF and CD34 by the ultrathin sections of brain tissue and the method of immuno histochemistry technology : After the six groups rats had the medicine or substitute medicine and then disparity has significantly disparity between DXTLD high dosage group and I/R group(P<0.05). Videlicet:DXTLD could obviously increase the VEGF and CD34 expression in cerebral cortex and hippocampus and relieve nerve cell injury.Conclusions:DXTLD can protect brain tissue of cerebral ischemia reperfusion injury and improve the nerve function. The mechanism may be related with the increasing of VEGF expression and Cd34 expression,and accelerated the vascularization of flap,established lateral branches circulation, improved brain ischemic injury as the ischemia continued.