The Study On Cerulenin-induced Osteosacoma Cell Line U2-OS Apoptosis Via Mitochondria Pathway

Objection:In order to explore the possible signal transduction pathway involved in the apoptosis induced by cerulenin in osteosarcoma cell line U2-OS.Methods:Western blotting was used to determine the expression of apoptosis-related protein bcl-2 and bax, pre-and after treatment with different concentrations of cerulenin.â‘ Osteosarcoma cell line U2-OS were treated with various concentrations of cerulenin (2.5mg/l,5.0mg/l,10.0mg/l,20.0mg/l)for 24h;â‘¡Osteosarcoma cell line U2-OS were treated with 5.0mg/l ( according to the IC50 from MTT experiment)cerulenin for 24h,48h,72h indivisually. Then we used single decontamination cell lysate to extract protein from cells, a spectrophotometer was used to detect the total protein level, equivalent samples were added for the western blotting.Results:With increased concentration of cerulenin (2.5mg/l,5.0mg/l,10.0mg/l,20.0mg/l),the expression of bcl-2 in Osteosarcoma cell line U2-OS decreased gradually, while the expression bax increased. 5.0mg / l Cerulenin treated the osteosarcoma cell line U2-OS for 24h, 48h, 72h respectively, with the extension of time, the expression of bcl-2 was gradually reduced, and bax had a gradual increase in the expression.Conclusion:Bcl-2 and Bax protein expressed positively in Osteosarcoma cell line U2-OS; The expression of Bcl-2 in Osteosarcoma cell line U2-OS was down-regulated with increased concentration of cerulenin or the extended treatment, and the expression of Bax was up-regulated with increased concentration of cerulenin or the extended treatment. We suggested that cerulenin induced the apoptosis of Osteosarcoma cell line U2-OS through the mitochondrial pathway, for the investigation that cerulenin could influence the expression of Bcl-2 and Bax.