Expression Of Tissue Factor In Prostate Cancer And Its Clinical Significance

Objective To study the expression of tissue factor (TF) in prostate cancer (PCa) and discuss its significance in clinic.Methods TF mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in 10 cases of human PCa tissue specimen and benign prostatic hyperplasia(BPH) tissue specimen. TF expression were studied immunohistochemical method in specimens of 30 cases of PCa and 20 cases of BPH .Microvessel density(MVD) was evaluated using anti-CD34 antibody as an endothelial marker with the same technique as well. Then the relationship between TF expression and clinic pathological data were analyzed.Results (1) The relative mRNA expression intensity of TF in PCa tissues were 0.48±0.30,those were significantly higher than that in BPH tissue(F=10.66,P<0.05). (2) In cases of benign prostatic hyperplasia (BPH), low grade prostate cancer (LGPCa), and high grade prostate cancer (HGPCa) tissue, the mean value of MVD were 25.35±3.79,45.80±5.71 and 68.70±6.47 respectively,MVD was positively correlated with histological grading (F=324.37,P<0.05). (3) Expression of TF was significantly higher between PCa and BPH group (x2=17.95, P<0.05), and it was positively correlated with the malignancy of PCa pathological grade. (4) Expression of TF was positively correlated with MVD and PSA (rMVD=0.915, rPSA=0.819 P<0.01). (5) Expression of TF was positively correlated with local infiltration, lymph node metastasis and bone metastases, while was no significance with age classification and whether or not prostate nodules in this group.Conclusions TF might play an important role in development and progress of PCa, which expressed specifically and stablely in PCa tissue, and it may provide a theoretical basis for targeting for immunotherapy prostate cancer.