Effects Of Pollen Typhae On The Atherosclerosis Rat Models

Background:Pollen typhae has obvious effect of activating blood circulation to dissipate blood stasis and diuretic in traditional Chinese medicine theory. Modern pharmacology verified that Pollen typhae could adjust the serum contents of lipids, protect endothelial cell and prevent the progression of atherosclerosis by different ways.Dyslipidemia has important significance in the progression of atherosclerosis. The functional defect of low density lipoprotein receptor (LDLR) gene is one of the main reasons that lead to hyperlipidemia and atherosclerosis. Study showed that LDLR in dyslipidemia patients was defective in function and injured in different degree, which resulted in the plasm levels of low density lipoprotein increasing and atherosclerosis. NO (nitric oxide) is accepted as one of the markers of endothelium function. NO could prevent the progression atherosclerosis by different ways. MDA (malondialdehyde) is a lipid peroxidation product. The levels of MDA increasing can make endothelial cell damage, and promote the formation of atherosclerosis. Our experiments plan to observe the effect on the serum contents of lipids, NO, MDA, and the effect on low density lipoprotein receptor gene expression with Pollen typhae.Methods:1. Pollen typhae suspension was prepared.2. Sixty Wistar rats were divided at random into five groups: normal group, model group, low-dose Pollen typhae group, medium-dose Pollen typhae group, high-dose Pollen typhae group. The later four groups were injected vitamin D3 in belly cavity at the first day of experiment and fed with high-fat forage. Normal group were treated with normal saline at the same volume in same way and standard forage. Pollen typhae groups were separately fed with different concentrations of Pollen typhae suspension every day.3. Morphological changes of aorta were observed under gross and light microscope after six weeks and eight weeks. The contents of total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol, nitric oxide and malondialdehyde in serum were examined. The changes were analyzed of LDLR gene expression in rats'liver by RT-PCR.Results:1. The total cholesterol, triglyceride, high density lipoprotein cholesterol, and low density lipoprotein cholesterol contents in blood serum The levels of serum total cholesterol, triglyceride, and low density lipoprotein cholesterol were higher in model group compared with normal group(P<0.05).High-dose Pollen typhae suspension decreased the levels of serum total cholesterol,triglyceride,low density lipoprotein cholesterol(P<0.05),while low-dose Pollen typhae suspension and medium-dose Pollen typhae suspension didn't.2. The nitric oxide and malondialdehyde contents in blood serumThe contents of nitric oxide and malondialdehyde in blood serum were higher in model group compared with normal group(P<0.05)).Three doses Pollen typhae decreased obviously the contents of malondialdehyde in blood serum(P<0.05).The contents of nitric oxide in blood serum weren't obvious difference between Pollen typhae groups and model group.3. The effect on low density lipoprotein receptor gene expression with Pollen typhaeThe low density lipoprotein receptor gene expression decreased markedly in model group compared with normal group(P<0.05)).High-dose Pollen typhae suspension markedly enhanced low density lipoprotein receptor gene expression(P<0.05).Conclusion:High-dose Pollen typhae suspension can enhance low density lipoprotein receptor gene expression in rat's liver, and finally accomplish the purpose of reducing serum lipids, regulating its metabolic disorder and preventing the development of atherosclerosis.