The Significance In Expression Of HPV L1 Capsid Protein In Liquid-based Thiner Cytology To Cervical Cancer Screening

Objective:To research the expression of the key capsid protein of human papillomavirus(HPV L1) in abnormal liquid-based thiner cytology,at the same time, combine the results of biopsy histology to approach the relationship between L1 protein and cervical neoplasia.So that we can evaluate whether it can be used as a subsidiary diagnostic marker for cervical cancer screening and provide a more specific method for the early diagnosis of cervical carcinoma.Methods:Selecting 50 patients whose LPT results were above atypical squamous cells of undetermined significance and hc2-HPV detect were positive,and 10 normal patients whose hc2-HPV detect were negative to make liqui-prep smears and to be done cervical biopsy under the colposcope.Using immunocytochemical streptaridin-peroxidase(S-P) method and non-isotope-labeled nucleic acid hybridization to test the L1 protein on 60 liqui-prep smears.At the same time, compared with the results of biopsy histology.Results:1.L1 protein were no expression in the control group.In patients with cytological diagnosis of atypical squamous cells of undetermined significance (ASC-US),low grade squamous intraepithelial lesion(LSIL),and high grade squamous intraepithelial lesion(HSIL),the positive rates of L1 protein were 76.2%(16/21),68.4%(13/19),10%(1/10),respectively.The positive rate in ASC-US or LSIL was significantly higher than NILM,HSIL,but unmarked difference bteween the other group.2.Compared with the results of biopsy histology,the control group pathologic result were chronic inflammation.The pathologic diagnosis results of remaining 50 patients were chronic inflammation,CIN-1,CIN-2,CIN-3 and SCC,which's positive rates of L1 protein were 84.6%(11/13),81.3%(13/16),50.0%(5/10),14.3%(1/7), 0.0%(0/4),respectively.The positive rate of the groups above CIN-2 was 28.6%(6/21). The positive rate in chronic inflammation or C1N-1 was significantly higher than the control group,and the groups above CIN-2,but unmarked difference bteween the other group.Conclusion:1.The expression rates of L1 protein were significantly different in different cytopathology diagnosis,the more severe lesions,the lower expression rates.2.L1 protein-negative has some diagnostic value to high level cervical lesions, which can be used as a subsidiary diagnostic marker for cervical cancer screening.3.L1 protein reflect the status of HPV infection in a certain extent.4.Liqui-prep samples adapt to do immunocytochemical analysis and nucleic acid hybridization.We believe it supplies a useful plat to research the variety biomolecules of cervical lesion.Using L1 protein in LPT smear will help our clinical doctors to find out high level cervical lesions.