The Expression And Significance Of Skp2, E2F1 In Difference Stage Of Hypertrophic Scar

Objective: To study the relationship that the expression of skp2, E2F1mRNA and protein and cell cycle of fibroblasts at the different stages of hypertrophic scars, in order to provide an initial basis research about gene interference for future prevention and treatment of hypertrophic scars. Methods: The samples of hypertrophic scars(n=8 in each group) were collected respectively from hypertrophic scars of 3-month, 6-month, 1-year,, 2-year and normal skin. The expressions of skp2, E2F1 mRNA and protein were detected by RT-PCR and Wetern Blot.. And at the same time, the flow cytometer was used to detect the fibroblasts cell life cycle. Results: (1) With development of HS. the expression mRNA and protein of skp2 and E2F1 in fibroblasts of hypertrophic scar experiences from high to low level,, and that of in 3 months group and 6 months group was higher respectively than that of 1years, 2 years and normal group (P <0.05) , and that of in 1 year group, 2 years group and normal group were no difference (P>0.05); The expression intensity mRNA and protein of skp2 in 3 months group was higher than that of 6 months (P <0.05), The expression intensity mRNA and protein of E2F1 in 3 months group and 6 months group was no difference (P>0.05). (2) Results of flow cytometry: Most FB were in S and G2/M stage (cycle) in 3 months,6months group; And most FB were in G0/G1 stage (cycle) in 1 year , 2 years group and normal group. the rate of FB in S and G2/M stage (cycle) were no difference in 3 month and 6 month groups(P>0.05), and that in 3 months group and 6 months group were different that of 1years, 2 years and normal group(P <0.05 ), lyear group and 2years, normal group were difference(P <0.05), 2years and normal group was no difference(P>0.05). The rate of FB in G2/M: 3 months group and 6 months group was no difference(P>0.05); 3 months group and 6 months group was lower respectively than that of 1years, 2 years and normal group(P <0.05); 1year group was lower than 2years and normal groups . 2years and normal group was no difference(P0.05). Conclusion: (1) The abnormal expression of cell cycle regulatory gene skp2 and E2F1 are Closely correlation with the development of hypertrophic scar. (2) The mRNA and protein expressions of skp2 and E2F1 in different stages of hypertrophic scar are basically consistent, And the distribution of fibroblastic in cell life cycle match to the function of skp2 and E2F1. (3) The high percentage of fibroblastic cells at S and G2/M in esrly stage of hypertrophic scar suggest that an interventing in early stage be an important target to the genesis and development of hypertrophic scar.