The Expression And Clinical Significance Of FOXM1B In Hepatocellular Carcinoma And The Relationship With MTA1 And MMP-2

OBJECTIVE: To explore the biological and clinical significance of FOXM1B and the relationship with MTA1 and MMP-2 in human hepatocellular carcinoma(HCC), through determining the expression level of mRNA and protein on the samples from HCC patients.METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) was employed to determine the expressions of FOXM1B,MTA1 and MMP-2 mRNA in 60 HCC tissues and their adjacent liver tissues and 18 noncancer liver tissues. Immunohistochemisty (S-P methods) was employed to determine the expressions of FOXM1B, MTA1 and MMP-2 in 60 cases of HCC tissues and their adjacent liver tissues. Immunohistochemical results to judge the report refer to Xu Liang Zhong by cancer cells staining intensity and the percentage of the number of positive cells, staining intensity and percentage of positive cells multiplied by> 3 points counted as immunoreactive. And the expression of gene and protein and the relationship of clinicopathological parameters were analyzed.RESULTS: The expression positive rates of FOXM1B mRNA,MTA1 mRNA and MMP-2 mRNA in the HCC tissues (76.67%,71.67%,71.67%) were markedly higher than the adjacent liver tissues(38.33%,43.33%,35.00%, P<0.01),but the adjacent liver tissues were also markedly higher than in the noncancer liver tissues (16.67%,0,11.11%, P<0.01).The expression of FOXM1B mRNA in the human HCC tissue was significantly correlated with clincal stage, the presence of extrahepatic matastasis, the recurrence of tumor, the degree of tumor differentiation and the number of tumor. The expression of MTA1 mRNA in the human HCC tissue was significantly correlated with clincal stage, the presence of extrahepatic matastasis, the recurrence of tumor and the number of tumor. The expression of MMP-2 mRNA in the human HCC tissue was significantly correlated with clincal stage, the presence of extrahepatic matastasis, the recurrence of tumor, the degree of tumor differentiation and the number of tumor. The clinical stageâ…¢of FOXM1B mRNA expression in HHC was significantly higher than stageâ… -â…¡(P <0.01); HCC with the presence of extrahepatic matastasis was significantly higher than non-matastasis (P <0.01);recurrence after surgery was higher than those without recurrence (P <0.05); tumor differentiation gradeâ…¢-â…£was significantly higher thanâ… -â…¡grade (P <0.01); tumor number greater than 1 were higher than the single tumor (P <0.05).The positive rates of FOXM1B, MTA1 and MMP-2 in the HCC tissues were significantly higher than those in the adjacent liver tissues (65% vs. 31.67%, 61.67% vs.33.33%, 63.33% vs.28.89%, P<0.01). Upregulation of FOXM1B expression in the HCC tissue was significantly correlated with the clinical stage, the presence of extrahepatic metastasis, the recurrence of tumor and the differenti- ation degree of tumor, but not correlated with the number of tumor, the size of tumor and the level of alpha-fetoprotein (AFP) in serum. Upregul- ation of MTA1 expression in the HCC tissue was significantly correlated with the clinical stage, the presence of extrahepatic metastasis, the recurrence of tumor and the number of tumor, but not correlated with the size of tumor, the level of alpha-fetoprotein (AFP) in serum and the differentiation degree of tumor. Upregulation of MMP-2 expression in the HCC tissue was significantly correl- ated with the clinical stage, the presence of extrahepatic metastasis and the recurrence of tumor, but not correl- ated with the number of tumor ,the size of tumor, the level of alpha-fetoprotein (AFP) in serum and the differentiation degree of tumor.FOXM1B with MTA1 in human HCC tissues was positively correlated (r=0.787,P<0.001). FOXM1B with MMP-2 in human HCC tissues was positively correlated (r=0.858, P<0.001).CONCLUSION: The present data suggest that the aberrant expressions of FOXM1B,MTA1 and MMP-2 in HCC tissues are closely correlated to the tumor recurrence and metastasis of HCC, is possible to angiogenesis. Analyzing their expression may contribute to synthetically evaluating the prognosis of HCC.