Expression Of RECK In Carcinoma Of Endometrium And Its Relationship To MMP-2, MMP-9 And MVD

Background:Endometrial cancer is a malignant tumor of the female genitals, In recent years its incidence is rising. The main difference between malignant and benign tumors is that malignant tumors with invasive growth and to the characteristics of distant metastasis. Endometrial cancer growth and invasiveness of tumor cells to distant metastasis mainly through the degradation of extracellular matrix, basement membrane and the formation of a large number of new blood vessels to complete, while the matrix metalloproteinase, and RECK gene in this process play an important role.Matrix metalloproteinases are a class of proteolytic enzymes, which can degrade the extracellular matrix, in which the most important are MMP-2, MMP-9, MMP-2, MMP-9 is a gelatinase (iV collagenase) class, which were originally is secreted when the inactive zymogen based on the form has been activated may play a role, its role consists mainly of two aspects: on the one hand by cancer cells to extracellular matrix and basement membrane degradation is conducive to cancer cells to other parts of the proliferation of walk, to promote tumorigenesis and development; the other hand, due to extracellular matrix and basement membrane degradation, for the formation of tumor blood vessels provide a space,And participate in the release of vascular endothelial growth factor in such processes, thereby promoting tumor angiogenesis, the growth of tumor development has provided an adequate blood supply to cancer cells enhanced invasion and metastasis.RECK gene is a tumor suppressor gene, a MMP inhibitor, can inhibit expression of matrix metalloproteinase-2 in blood vessel formation to invasion and metastasis of malignant tumors is inhibited.Many studies reported that played RECK gene on MMP inhibition, mainly for them to play a iV collagenase inhibition. Studies have shown that in lung cancer, colon cancer and many other malignancies, theRECK and MMP-2, MMP-9 expression and MVD counts relevant, were negatively correlated, so guess how much the amount of RECK protein expression with invasion and metastasis of malignant tumors was negatively correlated, RECK gene expression in patients with a better prognosis of high, so that the RECK gene in invasion and metastasis of malignant tumor inhibition. Many studies have shown that, compared with normal endometrial tissue, MVD in endometrial cancer tissues was significantly higher count, and clinical staging of endometrial carcinoma and the degree of differentiation-related, stage more nights, the lower the degree of differentiation, MVD count higher, suggesting the expression of MVD in endometrial cancer.The occurrence and the process of invasion and metastasis play an important role, and the higher the MVD count, their invasion and to the greater likelihood of distant metastasis, the higher its degree of malignancy, the worse the prognosis. Therefore, through the joint detection RECK and MMP-2, MMP-9 and MVD of endometrial cancer as a basis for the diagnosis of early diagnosis, but also by measuring the microvessel density as a determining prognosis of endometrial cancer, one of the indicators, and can be by inhibiting the formation of new blood vessels to serve the treatment of endometrial cancer to provide new ideas, worthy of clinical research applications.Objective:By endometrial carcinoma and normal endometrial tissue in the RECK gene and MMP-2, MMP-9 and MVD expression for detection and comparison to explore its expression in endometrial carcinoma characteristics and further analysis in endometrial cancer tissues RECK and MMP-2, MMP-9 and MVD relationship.Method:Strept avidin-biotin-horseradish peroxidase (SP) of 50 cases of endometrial cancer tissues,30 cases of normal endometrial tissue RECK and MMP-2, MMP-9 and MVD in expression for testing.The results:MMP-2, MMP-9 and RECK in endometrial carcinoma and normal endometrial tissues are expressed, In endometrial carcinoma, the positive expression rates were as follows:74.0%,68.0%,46.0%, In normal endometrial tissue in the positive expression rate:20.0%,30.0%, 80.0%, MMP-2, MMP-9 in endometrial cancer tissues was significantly higher than normal endometrial tissue, RECK in endometrial cancer tissue expression was significantly lower than normal endometrial tissue, the difference was significant (P<0.05); RECK protein in well-differentiated and medium-poorly differentiated endometrial cancer tissues were 55.6%,34.8%,Difference between the two was no significant (χ2= 2.1576, P= 0.142). RECK protein inⅠPhase,Ⅱ-Ⅳendometrial cancer tissues were 69.2%,20.8%, compared with the difference between the two significant(χ2= 11.7681, P= 0.001). RECK protein in myometrial invasion in endometrial carcinoma group of shallow and deep myometrial invasion group, the expression rates were 66.7%,15.0%, the difference between the two was significant (χ2= 12.8959, P= 0.000). In endometrial carcinomas, RECK protein expression and MMP-2 protein, MMP-9 protein expression was negatively correlated, (r=-0.424, r=-0.470, P<0.05). MVD counting in endometrial cancer tissues expressed as 15.9640±2.3780, in normal endometrial tissue as 8.9733±1.9203, the difference between the two was significant (P<0.05). MVD expression in I endometrial carcinoma,Ⅱ-Ⅳendometrial cancer tissues were 15.2846±1.9475,16.7000±2.6150, compared with the difference between the two significant (t=-2.182, P= 0.034). In endometrial cancer tissues of different levels of classification, MVD in-poorly differentiated endometrial cancer, endometrial cancer is high and the increased expression of differentiation, namely,16.4957±2.3984,15.5111±2.3081, no significant difference between the two sex; MVD in the superficial myometrial invasion in endometrial cancer group and the group expressed deep myometrial invasion were 15.2600±2.1226,17.0200±2.3982, between the two There was significant difference; MMP-2 expression and MVD positive phase. (R= 0.513, P= 0.000), MMP-9 protein and MVD count also showed a positive correlation (correlation coefficient r= 0.574, P= 0.000), RECK expression and MVD was negatively correlated (r=-0.747, P= 0.000).Conclusion:1. RECK in endometrial cancer tissue was low expression,and its low expression or no expression enhanced the invasion and metastasis.2. MMP-2, MMP-9 in endometrial cancer tissues were significantly increased, and its high expression in endometrial carcinogenesis closely with the occurrence of endometrial cancer and development.3. Microvessel density (MVD) in endometrial cancer tissues was significantly higher, and MMP-2, MMP-9 in endometrial carcinoma tissues were positively correlated with the MVD counts, which showed that MMP-2, MMP-9 in the promotion of high expression in endometrial carcinoma formation of new blood vessels.4. In endometrial cancer tissues RECK expression reduced, while microvessel density (MVD) expression was significantly increased, and RECK protein expression and MMP-2, MMP-9 and microvessel density (MVD), expression into the negative correlation, suggesting that we can through enhanced expression of RECK protein, inhibit the formation of new blood vessels to suppress the occurrence of endometrial cancer and development, and can be developed based on this two links related to new drugs for the treatment offers a new way of thinking.