Expression Of RECK And MMP-9in Endometrial Carcinoma And Its Clinical Significance

Objective Study the expression of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) and matrix metalloproteinase-9(MMP-9) in different endometrial tissues and probes into their roles of invasion and metabasis in endometrial carcinoma.Methods The expression of RECK protein and MMP-9protein were detected in42cases of endometrial carcinomas,15cases of atypical hyperplasia and26cases of proliferative endometrium by S-P immumohistochemical technique.Real-time quantitative fluorescence PCR were used to examine the expression of RECK mRNA and MMP-9mRNA in the three groups. The correlations between RECK and MMP-9expression in endometrial carcinomas were assessed by Pearson Chi-Square test.Results (1) Compared to atypical hyperplasia tissue and proliferative endometrium tissue,the positive rate of RECK protein and RECK mRNA in endometrial carcinomas group was significantly lower (P<0.05); MMP-9protein and MMP-9mRNA was significantly higher (P<0.05);(2) In endometrial carcinomas; both the expression rate of RECK and MMP-9gene was related to histologic grade, FIGO stage, depth of myometrial invasion and lymph node metastasis (P<0.05).(3) Spearman rank correlation analysis showed that the RECK protein and MMP-9protein expression existed a significantly negative correlationship(r=-0.599,P<0.01).Conclusions The low expression of RECK and high expression of MMP-9may have relationships with occurrence, development and metastasis of endometrial carcinoma. Both play an antagonist role in the invasion and metastasis of endometrial carcinoma.United detection of RECK and MMP-9by may have a certain value for predicting risk of early diagnosis of endometrial cancer and precancerous lesions.