Extraction And Purification Activity To Geranium Wilfordii Maxim Of Tannins And Preparation Floating Tablets

Geranium wilfordii Maxim component of the Chinese medicine has the ability of reducing rheumatism, smoothening energy channel, curing durchfall and so on. Geranium wilfordii Maxim includes main chemical compositions such as tannins, volatile oils, organic acids, in particular, the tannnins are rich in it. Study suggest that the extractive tannins in Geranium wilfordii Maxim has the effective ability of antiulcer.To optimize the extraction and purification process of tannins from Geranium wilfordii Maxim component of the Chinese medicine, and formulate the quality standard of extractive. Based on the major ingredients from extractive of Geranium wilfordii Maxim Tannins component, Preparation for the gastric-floating tablets, confirms the prescription and method, and establishes the quality standard.By the adoption of ultrasonic and circulated extraction process, the extraction rate of the Tannins component has increased obviously. The optimal parameters were obtained as follows: dosage liquor ratio was 1:12; the solvent was 40% alcohol; the extraction temperature was 20℃; the extraction time was 1 h; the interval time of the extraction time was 0.6:0.5 s; the ultrasoud power was 500 W.The general Tannins of gelatin sediment and acetone solution make sediment dissolve and release tannins putrefy the geranium extract preliminary. The optimal parameters were obtained as follows:the volume of Geranium wilfordii Maxim extractation was ten times than 4% gelatin solution and the optimal solvent was 50% acetone.Macroporous resins HPD100 purification parameters were obtained as follows:diameter and height ratio was 1:5, sample solution was 50 mL (1 mL sample equals 30 g raw Geranium wilfordii Maxim), cleaning by 500 mL distilled water and 250 mL 15% alcohol, then eluting by 250ml 70% alcohol in 1.5 BV·h-1.The research for extraction quality standard:gallic acid is control article, uses TLC and HPLC for qualitative identification and content mensuration to Geranium wilfordii Maxim extract.The prescription for Geranium wilfordii Maxim tannins of gastric-floating tablets was obtained as follows:HPMC:PVPP:MCC:NaHCO3(0.4:1.5:0.3:0.2:0.24); the average weight of the tablet is 150.2 mg, initial floating time<3 min, continued floating time>8 h, 4 h cumulating releasing rate>50%,8h cumulating releasing rate attained to 75%.The extraction and purification process for Geranium wilfordii Maxim tannins is steady and available, the quality of extract is controllable. The gastric-floating tablets is made by material from Geranium wilfordii Maxim tannins possesses the characteristics of gastric-floating tablets.