Effect Of Salvia Miltiorrhiza Bge On The Expression Of Bcl-2 And Bax After Facial Nerve Axotomy

Facial nerve injury is a common clinical problem in man. This is in part due to its long anatomical course in the cranium and its rather superficial location after its exit from the skull. Facial nerve injury results in facial muscular atrophy, which influences people's social interaction, as well as causes a loss of facial motoneuron in facial nucleus, which is one reason making facial neurotization difficult. Recent studies have shown that facial nerve injury Caspase family as an important effector of apoptosis implementation. The family may be at least as a chain activated, cyclic amplification and heterologous oligomerization are three ways to participate in apoptosis, through the destruction or damage to activate certain enzymes cytoskeleton, etc. led to a series of apoptosis-related responses and morphological changes. In which bcl 2 gene family expression product is an important apoptosis regulatory proteins, including bcl-2 gene (B-cell lymphoma/leudemia 2 gene) inhibit apoptosis, while bax (bcl associated x gene) to promote apoptosis, 2 those who are in dynamic balance, a balance of results determine cell survival or death. Bcl-2 inhibits cell apoptosis might be related to the role with the following: 1, cell antioxidant 2. Inhibit transmembrane calcium flow of 3. Control of the cell signal transduction pathwayDanshen (Salvia miltiorrhiza Bge) dicotyledons Lamiaceae. Indications: blood circulation to regulate menstruation, blood stasis and relieve pain, cooling blood elimination carbuncle, Qingxin Chufan, nourishing and soothe the nerves. But also to promote blood circulation, expansion of coronary arteries to increase blood flow, prevent platelet aggregation, protection of myocardial ischemia. Side effects: taking incorrectly can cause serious bleeding, resulting in life-threatening. Study confirmed that Salvia has the microcirculation, anti-lipid peroxidation, scavenging free radicals, preventing calcium overload, inhibit neuronal apoptosis and so on. Salvia on cerebral ischemia-reperfusion nerve cells Expression of Bcl-2 mRNA can raise the effect, so that the entire cerebral ischemia bcl-2 immunopositive cells significantly increased, and thus play its neuroprotective effect.This issue is guided by mentor Professor Zhang Wei experimental study carried out in sequence as part of this study is about the Salvia facial nerve injury. In the early experiments, we have proved that Danshen injection can effectively inhibit neuronal apoptosis facial, facial nerve injury and repair in order to play an active role, but does not address the specific mechanisms. The issue is following the previous experimental results, through the adoption of animal experiments as well as the immunohistochemical method of Danshen on rat neurons after facial nerve injury in bcl-2, Bax protein expression, to further explore Danshen injection on the possible mechanism of facial nerve injury, in order to provide guidance for clinical and reference.Wistar rats were 32, regardless of gender, body weight 250-300g. Were divided into experimental group (each preoperative 30min Danshen injection 300mg/kg, intraperitoneal injection); the control group (given saline). 30 rats the right facial nerve in a neck hole will milk facial nerve transection. The left facial nerve intact and did not make disposal. Salvia given immediately after the experimental group (50mg/kg, intraperitoneal injection), control group 2 given the same dose of saline, and so repeat (2 times / d), until the animals were killed. The other two rats without any disposition as a blank control.Patients at different time points (3,7,14,21,28 days) rats were killed, including the facial nucleus of the brainstem consecutive coronal sections, using immunohistochemistry to detect facial nucleus where bax, bcl-2 expression; Light microscopic calculation of the number of surviving neurons. 1, facial nerve transection injury, the SM group compared with the saline group, 7 days, 14 days, 21 days, 28 days Danshen group on motor neuron survival was significantly higher than saline group, suppression of the facial nerve motor neuron death; 2 , nerve injury bcl-2 and Bax were expressed, bcl-2 expression gradually reduced, Bax expression increased gradually, Danshen can increase the expression of Bcl-2, inhibit Bax expression, increased Bcl-2/Bax ratio, Danshen group and the saline Compared with high levels of Bcl-2/Bax, reduce neuronal death, thereby enhancing the facial nerve injury survival motor neuron; Conclusion. In the facial nerve injury, Salvia can make facial nucleus in bcl-2 and Bax in dynamic equilibrium tends to inhibit neuronal apoptosis results, thereby increasing the facial nerve motor neuron survival.