| Objective: This article aims to research Livin and Smac protein in normal ovarian tissues, benign ovarian tumor and ovarian epithelial carcinomas, and study the expression level of their respective with epithelial oarian cancer of the clinical and pathological features, study the correlation between the two,to explore in epithelial ovarian cancer, the role of development for the diagnosis of epithelial ovarian cancer to determine the degree of malignancy and prognosis of theoretical basis, and for the further study of epithelial ovarian cancer gene therapy and solving the problem of tumor cell resistance provide a new theoretical basis.Methods: Randomly selected and The China-Japan Union Hopsital of Jilin University in January 2008 -2009 year in October between the surgical resection specimens. All specimens were fresh drawn immediately after 10% neutral formalin-fixed, paraffin-embedded. Serial sections of 4μm thick, each prepared three different cases, respectively, for HE staining, Livin and Smac immunohistochemical staining Immunohistochemical staining reagent positive film box known as a positive control, all the slices were identified by pathology specialists blinded . Immunohistochemical method of the organizations staining to detect the expression of Livin and Smac.Results: 1, Livin protein was mainly expressed in the cytoplasm, occasionally in the nucleus, which in normal ovarian tissues, ovarian benign organization, epithelial ovarian cancer tissue expression rate of the order of 5.0%, 15.0%, 65.0%, among the The positive expression rate of a very significant difference, there are statistically significant (χ2 = 30.03, P <0.05). Epithelial ovarian cancer tissues and normal ovarian tissue in the expression of Livin significant difference (χ2 = 21.60, P <0.05); the same time, and benign ovarian tumor tissues compared with the expression of Livin also significant differences (χ2 = 15.07, P <0.05) ; while the normal ovarian tissue and ovarian benign tumor tissues of Livin expression was statistically significant (χ2 = 0.28, P> 0.05).2, Smac protein was mainly distributed in the cytoplasm, occasionally in the nucleus, and its normal ovarian tissue, ovarian benign tumor tissues followed by expression rate of 90.0%, 85.0%, 56.7%, among the three groups, the difference was significant , there are statistically significant (χ2 = 10.78, P <0.05). Comparison between the two groups, ovarian epithelial carcinoma and normal ovarian tissue in the expression of Smac significant difference (χ2 = 7.33, P <0.05); the same time, and benign ovarian tissues there was a marked difference compared to (χ2 = 5.21, P <0.05); however, benign tumors and normal ovarian tissue and ovarian tissue expression of Smac had no significant statistical significance (χ2 = 0, P> 0.05).3, Livin protein in highly differentiated epithelial ovarian cancer group, in the differentiation group and the expression rate of poorly differentiated group were: 43.8%, 50.0%, 81.3%, comparison between the three groups,χ2 = 8.08, P <0.05, Livin there is significant difference in the expression. In the surgical-pathological staging of I, II and III, IV period of positive expression rates were 56.7%, 73.3%, no significant difference between the two, no significant statistical significance (χ2 = 1.83, P> 0.05). In the mucinous cystadenocarcinoma, serous cystadenocarcinoma, endometrioid carcinoma of the expression rate of the order of 68.0%, 60.0%, 70.0%, Livin expression in different histological types, no significant difference was not statistically significant (χ2 = 0.48, P> 0.05).4, Smac protein in well-differentiated epithelial ovarian cancer group, in the differentiation group and the expression rate of poorly differentiated group were: 75.0%, 75.0%, 40.6%, compared among the three groups,χ2 = 7.19, P <0.05, its expression there is significant statistical difference. In the surgical-pathological staging of I, II and III, IV period of positive expression rates were 70.0%, 43.3%, both compared to,χ2 = 4.34, P <0.05, there was a significant difference, there are statistically significant. In the ovarian epithelial cancer histologic type mucinous cystadenocarcinoma, serous cystadenocarcinoma, endometrioid carcinoma of the expression rate of the order of 56.0%, 64.0%, 40.0%, compared the three,χ2 = 1.68, P> 0.05 , the difference was not significant, no clear statistical significance.5, in 60 cases of epithelial ovarian cancer tissues the expression of Livin protein expression of Smac protein-negative accounted for 24 cases, Livin protein negative expression of Smac protein expression were a total of 19 cases, while the positive expression of both, in 15 cases, while were two cases of negative expression, showing that in epithelial ovarian cancer when the Livin protein expression showed low expression of Smac protein in many states, but Livin negative expression of protein when the high level of Smac protein expression by Spearman rank correlation analysis showed that both negative related, r = -0.501, P <0.01.Conclusion: 1. Livin expression may contribute to the high incidence of ovarian cancer development.2. Smac expression may contribute to the low incidence of ovarian cancer development.3. Livin in epithelial ovarian cancer protein may indicate the malignancy and poor prognosis, easy to relapse.4. Smac expression may indicate a low more aggressive tumors and poor prognosis.5.Livin and Smac gene are involved in epithelial ovarian cancer patho- genesis and progression of the cancer has some relevance, but they have opposite biological effects, there is a negative correlation, will combine the two, combined detection of diagnosis of epithelial ovarian cancer to determine the degree of malignancy and prognosis value...
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