| Objective:Gastric cancer is one of the most common malignacies in our country. The incidence and fatality rate is in the forefront of malignancies. Although recent progress of multidisciplinary therapy has improved the curative effect, the 5 year overall survival of gastric cancer patients is still unsatisfactory. It is realized that the failure of treatment can be attributed to multi-drug resistance and severe toxic response. Investigation of efficient, low-toxic drugs or treatments has important clinical significance. Traditional Chinese medicine is a treasure trove of our country in aspect of medicine. In the recent years, as the development of technology of elements extracting and pharmacological research, many kinds of elements such as Curcumin and Resveratrol extracted from the Chinese medicine were confirmed to have an effect to inhibit proliferation and induce apoptosis of tumor cells. And some have been applied to clinical, such as paclitaxel. Clematis is a large genus in the Ranunculaceae family, which is widely distributed in China and has many pharmacological actions. The roots and rhizomes of Clematis are called weilingxian in Chinese, which is commonly used as an analgesic, abirritative, antibacterial, antiphlogistic, anticancer and diuretic agent. It is investigated that some elements from Clematis has cytotoxic activity and inhibit proliferation in vitro. But the research is not deeply enough .We investigate in vitro the effect of a new triterpene saponins extracted from Clematis ganpiniana on growth and apoptosis of human gastric carcinoma cell line SGC-7901, and on NF-κB activation. Furthermore, we preliminarily explore its possible mechanism of NF-κB inhibition, which is helpful for our further study.Methods: 1.Human gastric carcinoma cell line SGC-7901 cells were treated with Clematis saponins and/or with 5-FU. MTT assay was used to evaluate the growth inhibition rates .Negative control and blank control were set. 2. Apoptosis rate of SGC- 7901 cells treated with Clematis saponins and 5 - fluorouracil (5-FU) was determined by Annexin V /PI double staining flow cytometry (FCM). 3. Western Blot was used to test the expression of NF-κB in nuclear. 4. The intranuclear activity of NF-κB was confirmed by ELISA assay.Results: 1.The inhibition rates on SGC-7901 cells in each Clematis saponins treatment group were significantly higher than those in control group (P < 0. 05), and the inhibition rates increased with the concentration increase of Clematis saponins. The growth inhibitory effect of 5-FU on SGC-7901 cells was significantly enhanced by Clematis saponins. 10μg/ml of Clematis saponins caused 23% inhibitory rate. The inhibitory rate of 5-FU was 28%. Combined treatment of Clematis saponins and 5-FU resulted in 48% inhibitory rate. The Clematis saponins could obviously promote on cell apoptosis induced by 5-FU. 2. Western Blot and ELISA confirmed that the Clematis saponins could significantly inhibit the expression and activity of NF-κB in nuclei. And the inhibition pattern showed in dose- dependent manners. 3. Clematis saponins had a promotion of 5-FU induced cell apoptosis. The apoptosis rate of SGC-7901 cells was of 8.3% in 5-FU treated group and of 12.3% in Clematis saponins treated group, and of 29.1% in combined treatment of the two agents as well (P<0.01).Conclusion: Clematis saponins can significantly inhibit proliferation and induce apoptosis of gastric carcinoma cell SGC-7901, which might be partly related to the inhibition of the expression of NF-κB. These data suggests that Clematis saponins, as a novel candidate of NF-κB inhibitor, might be of clinical significance in apoptosis-inducing cancer therapy.
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