Regulation Of Adiponectin MRNA Expression By Glargine, Detemir And Human Insulin In 3T3 - L1 Adipocytes

Objective: To investigate the effect of glargine,detemir and human insulin on the expression of adiponectin mRNA in 3T3 - L1 adipocytes.Methods: 1.3T3-L1 preadipocytes were cultured in vitro until they were differentiated into mature adipocytes,then they were cultured in basal medium for 24 hours to eliminate the influence of differentiation medium,at last they were cultured for 24 hours in basal medium which containing of 1nmol/L,10nmol/L,100nmol/L,1000nmol/L human insulin(powder),insulin glargine(powder and injection) , insulin detemir (injection ) ,and established basal medium group for control group, after 24 hours, the level of adiponectin mRNA in adipocytes were measured by real-time quantitative reverse transcription - polymerase chain reaction (RT - PCR ).2.Statistics analysis: Values are presented as mean±SE. Many groups of quantitative data were compared by one-way analysis of variance (ANOVA),multiple comparison used LSD-t test or Dunnet's method . A P-value≤0.05 was considered statistically significant.Results: 1.The effect of different concentrations but same insulins on the expression of adiponectin mRNA:different concentrations (1nmol/L, 10nmol/L, 100nmol/L, 1000nmol/L) of insulins acted on mature 3T3- L1 adipocytes for 24h, along with the increase of insulin concentration,adiponectin mRNA expression reduced gradually.The suppress effects of insulins on adiponectin expression were not significant ( P > 0.05 ) when the concentration≤10nmol/L.When the concentration of insulins was higher than 100nmol/L,the adiponectin expression was significantly suppressed(P<0.05).The inhibition of 1000nmol/L insulins were stronger than 100nmol/L insulins.2.The comparison of the effects of same concentrations but different insulins on the expression of adiponectin mRNA:there were no differences among groups when the concentration of insulins was 1～10nmol/L. When the concentration of insulins was 100nmol/L, 1000nmol/L,the inhibition of insulin detemir (injection) was weaker than insulin glargine(powder and injection) and human insulin(powder).The inhibition of insulin glargine(powder and injection) was weaker than insulin human(powder). There were no statistical differences between glargine insulin powder and glargine insulin injection.Conclusion:1. High concentration (100nmol/L,1000nmol/L)glargine,detemir and human insulin all inhibit adiponectin mRNA expression in 3T3 - L1 adipocytes with varing degrees,and in a dose-related manner.2. The inhibition of insulin detemir is weaker than insulin glargine and insulin human.The inhibition of insulin glargine is weaker than insulin human.