| [Abstrac] Objective:VX2 transplantation of rabbit in different periods in melanoma peripheral blood platelet activate marker, this paper discusses the monitoring, compared with liver cancer platelet activate the close relationship between the platelets and activation in liver cancer occurrence, development, and the important role of infiltration, transfer of hepatocellular carcinoma. For clinical diagnosis, treatment, illness evaluation, etc, providing new theoretical basis from blood clotting gets more treatment, and to further improve the level of diagnosis and treatment of HCC patients. For clinical liver cancer diagnosis, treatment, illness evaluation, etc, providing new theoretical basis from blood clotting gets more treatment, and to further improve the level of diagnosis and treatment of HCC patients. For clinical liver cancer diagnosis, treatment, illness evaluation, etc, providing new theoretical basis from blood clotting gets more treatment, and to further improve the level of diagnosis and treatment of HCC patients.Methods:1. Take New Zealand big white rabbit, only 60 randomly divided into (1) liver model group; (2) JiaShouShu group; (3) normal control group.2. Tumor block anesthesia preparation:will a tumor-burdened rabbit, sterile conditions stripping tumor, cut, get close to capsule tumors RouYang organization in gray fish saline (including gentamycin 2 million U), eliminate necrotic tissue and fiber organization, will tumor tissue shear ground into 1-2mm3 small pieces. Set aside.3. tumor cells levitation liquid preparation:tumor block+ physiological saline made suspension liquid 1ml about 5x, the concentration of 1010 a/L.4, planting rabbit VX2 transplant tumor model sigmoidectomy puncture inoculation method adopted left lobe, will pull in vitro liver needle injection, with 18G (spare tumor 3-4 organization pieces) or tumor cells levitation liquid 1ml spear pierced depth about 2cm, pull needle with 1cm size gelatin sponge after 2 minutes, will block oppression liver back on, layered shut abdomen, successful planting liver rabbit model 30 only; JiaShouShu group take sigmoidectomy shap, will direct the liver pulled outside, the left lobe with 18G needles into physiological saline, 1ml pinpoint Pierce depth about 2cm, pull needle with gelatin sponge block after 2 minutes, oppression, layered on liver back will be closed abdominal (n= 30); Normal controls normal and healthy New Zealand rabbits did not undertake any intervention large (n= 30).5.adopt flow cytometric profilometry (FCM), respectively on 6 h after planting,48 h, the comprehensive in 2w 1w leds, blood, monitoring the platelet activate markers CD62p expression level, compared with standard data mean±(x±s) says, compares the two sample mean t test, the group design with P< 0.05 for difference have statistical significance. After planting in expl 1w leds,2w death 15 rabbit liver, lungs anatomic observation without metastases, of femoral vein, portal without thrombosis within. Results:1, the research team peripheral blood platelet activation markers CD62p level obviously higher than those in the control group, and the transfer of tumor progression, platelet activate marker has risen,2 weeks of peak; 2.after planting anatomical rabbit, delayed-type 1w leds not seen transfer and thrombosis. Double lung and after 2w team are JiaShouShu liver metastases,2 cases of thrombosis, the normal group had not changed. Research group and control group, and compared using t-test between groups, (t= 0.18) (P < 0.01) difference was statistically significant. Conclusion:VX2 rabbit transplant tumor peripheral blood platelet is active with tumors, and the occurrence, development and transfer of platelet activate markers expression was increased. Platelet activate markers detection can be used as tumor progression, transfer effective evaluation index.
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