Expression Of TSPAN-1 Protein And The Relation With Ki-67,CD105 In Cervical Squamous Carcinoma Tissue

Cervical cancer is the most common malignant tumor in female genital system. The major route of metastasis of cervical cancer has two kinds:direct spreading and lymphatic metastasis. According to statistics, each year there are about 500,000 new cases of cervical cancer worldwide, about five percent of the cases. And eight percent of cervical cancer occurs in developing country. In china there are 135,000 fresh cervical cancer sufferer, hold about one third of the world's fresh sufferer and 80,000 Chinese women died of cervical cancer each year. Cervical cancer's formation rate also gradually presents the youth oriented and the rise potential state, The pathogenesis of cervical cancer is evolving from cervical intraepithelial neoplasia as a continuous pathological process, namely arrives at the cancer from the cervix atypical proliferation, ultimate development for infiltration cancer. The incidence of cervical cancer is related to HPV infection, sexual disorder, premature sexual activity, early childbirth, dense production, productive, economic status, race and geographical environment and other factors. And cancer cell proliferation, angiogenesis, cancer cell migration are closely linked to cancer's occour, development, metastasis. At present, experts begin to pay close attention to the tumor-suppressor gene, anti-metastasis gene and gene product protein, angiogenesis and other aspects. Finding advantageous indicatrix which can early diagnosedecide, pathogenetic condition progress, and estimate prognosis have great significance for healing and preventing cervical cancer.The tumor multiplies related molecular TSPAN-1 (tetraspanin-1) also to be called C4.8, NET-1, P503s, is four cross membrane protein super family's (transmembrane 4 superfamily, TM4SF) young blood which discovered by S.C. Todd in 1998. TSPAN-1 in many kinds of tumor organizations (for example breast cancer, cervical cancer, colon cancer, esophagus cancer, liver cancer, lung cancer, ovarian cancer, cancer of the pancreas, prostate gland cancer, stomach cancer) has had higher expression, possibly causes cancer tissue cell's multiplication and blood vessel's mechanisms by transducing cell division's signal and (either) causing the differentiation or the dedifferentiation, and so on participate the cancer's process and the attack transition process. Cell's unusual multiplication is one of important mechanisms in tumor occurs. Ki-67 exists generally in all humanity's multiplication cell nucleus, is one kind of multiplication cell related nuclear antigen and its function are closely related with mitosis. Ki-67 apply in determine each kind of tumor multiplication activeness, auxiliary distinguishes disease innate or malignancy. The tumor capillaries production is a key parameter which reflects the tumor malignant biology behavior, CD 105 is a kind of "membrane associativity glycoprotein" exists in the cell membrane surface, may take one kind of newborn blood vessel's characteristic value symbol. Only in the multiplication tumor organization's blood vessel bast have CD105's strong expression, it can accurately appraise blood vessel's generative capacity. Therefore CD105 display influential role in regarding malignant tumor diagnosis and prognosis.ObjectiveTo investigate the expression of TSPAN-1 proteins in cervical squamous carcinoma tissue and the dependablity of TSPAN-1 with cancer cell multiplication and capillaries density.Materials and Methods1 Study ObjectChosen 30 cases of cervical squamous carcinoma patients,30 cases of cervical intraepithelial neoplasia patients (CINⅡ14 cases, CINⅢ16 cases),30 cases of hysteromyoma patients who has normal cervix in the Third Affiliated Hospital of Zhengzhou University to enroll in this study. All these patients are all in 34-65 years old of scope and have non-hormone medication history and any immunity inhibitor 3 months before the technique, have not accepted the cytotoxic drugs and the radiotherapy. Collected 90 samples from operation. All specimens are fixed after 10% neutral formalin, embedded in paraffin wax conventional, made thick serial section to 4μm, and confirmed by pathological diagnosis. 2 MethodsBy immunohistochemical SP method detect expression levels of TSPAN-1,Ki-67,CD105 protein in 30 cases cervical cancer,30 cases precancerous lesions and 30 cases normal cervix tissue, analyze the dependablity of TSPAN-1, Ki-67, CD105 with clinical pathological of cervical squamous cancer, and the relation between TSPAN-1 and Ki-67,CD105.3 Statistical treatmentThe data were recorded and analyzed by SPSS13.0 package. The positive expression comparison of TSPAN-1 and Ki-67 protein deploy x2 analysis, and comparison between each pair groups deployχ2 diremption; The expression of CD 105 deploy (x±s), and it's comparison in groups deploy one-way mean square analysis (ANOVA), and comparison between each pair groups by least significant differerence analysis. Before mean square analysis should do Shapiro-Wilk Test and Levene's Test; the comparison of expression of TSPAN-1 and Ki-67 protein with clinical patho-factor deploy Fisher exact possibility; comparison of expression of CD 105 protein with clinical patho-factor deploy t analysis; The dependablity between TSPAN-1 and Ki-67 in cervical cancer deploy correlation analysis, The dependablity of TSPAN-1 with CD105 deploy t analysis. Significance level is a=0.05. Each pairs groups compare remedy significance level a=0.017.Results1 The expression of TSPAN-1 in the cervical tissure of different research object:In the process of normal cervix to the cervical intra-epithelial neoplasia to cervical squamous cancer, the expression of TSPAN-1 increase gradually (P<0.05).2 The expression of Ki-67 in the cervical tissure of different research object: The positive expression level of Ki-67 of in the normal cervix tissue is significant lower than it in the cervical intra-epithelial and cervical squamous cancer(P<0.017); But the positive expression level of Ki-67 in cervical squamous cancer have non-significant higher than it in the cervical intra-epithelial neoplasia (P> 0.017).3 The expression of CD105 in the cervical squamous tissure of different research object:In the process of normal cervix to the cervical intra-epithelial neoplasia to cervical squamous cancer, the expression of TSPAN-1 increase gradually (P<0.05).4 The correlation of expression of TSPAN-1 with clinical patho-factors in cervical squamous cancer:Significant difference of TSPAN-1 expression was found among the pelvic lymph node metastasis status (P<0.05).5 The correlation of expression of Ki-67 with clinical patho-factors in cervical squamous cancer:The expression levels of Ki-67 have non-significant difference among all the clinical patho-factors in cervical cancer (P>0.05).6 The correlation of expression of CD105 with clinical patho-factors in cervical squamous cancer:There existed significant differences in CD105 correlated with the different stages and the lymph node metastasis status (P<0.05).7 The expression level of TSPAN-1 and Ki-67 in cervical squamous cancer were positively correlated (r=0.539, P<0.05).8 The expression levels of TSPAN-1 and CD 105 in cervical squamous cancer were positively correlated (t=3.912, P<0.05).Conclusions1 In cervical squamous cancer the expressionof TSPAN-1,Ki-67 and CD 105 increased gradually. It prompt that TSPAN-1,Ki-67 and CD 105 have great correlation with cervical squamous cancer oncogenesis.2 The expression of TSPAN-1 and CD105 are closely related with aversion and prognosis appraisal of cervical squamous cancer.3 In cervical squamous cancer tissues, the expression of TSPAN-1 and Ki-67,CD105 were positively correlated. This hints that they may play function in coordination with each other, and TSPAN-1 may contribute to cervical squamous cancer's oncogenesis and development by participating tumor cell's multiplication and tumor angiogenesis.