The Expressions Of CD105, MK And VEGF In Colorectal Cancer Tissues And Their Clinical Singnificance

Background : Colorectal cancer is one of the common malignant tumors in gastric bowel path in China and its mobidity is still increasing.Although the operation-based comprehersive therapies have greatly improved the prognesis of colorectal cancer patients in recent years ,the postoperative 5-year survival rate ,unfortunately is still approximately 50-60%.At present,the key point of cutting down the case fatality rate and death rate of colorectal cancer is early detection,early diagnosis,early treatment.Early detection can judge effectually the progression and evaluate prognostic.As a bionmarker of vascular endothelial cells proliferation,CD105 stimulates vasculogenesis and plays key role in tumor angiogenesis.The study has confirmed the expression of CD105 positively correlates with the malignant grades,lymph node metastasis and recurrence incidence in a variety of tumors.MK has been many medical wokers and aberrant MK expression has been reported in variety of human tumors/cancer tissues.Recently,newly born tumor vessel's effect has aroused people's concern for the effluence,development,diversion and infiltration on tumor increasingly,furthermore,it has been a big hotspot of research.VEGF is the most representative of them. Therefore,further exploring the alterations and role of key genes related to effluence,development of colorectal cancer will be considered extremely useful for early diagnosis,treatment and prognosis.Objective To investigate the expression and significance of CD105,MK and VEGF in colorectal cancer.Materials and methods (1)Surgical specimens used for this study were obtained from 100 patients with colorectal cancer , which were confirmed pathologically and embedded in paraffin at hospital from Jan. 2002 to June.2003.All the samples including 60 colon cancer,40 rectum cancer and 20 normal mucosa specimens which were 10cm far colorectal cancer loci.(2) The expressions of CD105 , MK and VEGF in colorectal cancer tissued were studied by immunohistochemical staining.Results The mean vaule of MVD was 86.62士21.68,the positive expression rates of MK and VEGF in colorectal was 70.0% and 68.0% respectively.The value of MVD was apparently higher than in tumor tissue with both positive MK and VEGF than in that with both negative MK and VEGF ((108.24士22.21.VS 79.32士19.78).The expression of CD105,MK and VEGF were all colse correlated with T-stage and metastasis(lymph node invasion and distant metastasis):Triadic expression inⅢ,Ⅳgroup was higher than inⅠ,Ⅱgroup(P<0.05), Triadic expression in metastasis group was higher than in metastasis-free group.But the expression of CD105, MK and VEGF in colorectal cancer tissue are unrelated with age,gender and histological differentiation. The study revealed positive MK and VEGF made a big difference to a patient's 5-year survial on single-factor analysis ,however only the lymph node and liver metastases were independent prognostic factors on multivariate analysis.Conclusions (1) CD105 may be a promising tumor marker of colorectal cancer for diagnsis of colorectal cancer.(2)Colorcetal cancer with the development of vasculogenesis is closely-related.CD105.A combinative detcetion of CD105,MK and VEGF can be a valuable tumor marker and prognostic factor for colorectal cancer.(3)Both MK and VEGF are significantly related to clinical stage and metastasis( lymph node invasion and distant metastasis).In colorectal cancer ,the positive experssion of MK and VEGF is positive correlated.There may be some reciprocal induction or synergistic effect in regulating mechanism of tumor angiogenesis.MK and VEGF are involved in the process of the development and metastasis of colorectal cancer.(4)Inhibiting the expression of CD105,MK and VEGF might block the regression vascalogenisis of colorectal cancer,which may be a new pathway for treatment of colorectal cancer.