Effects Of Huanglianwendantang Decoction On Insulin Resistance And Blood Leptin In Metabolic Syndrome Rats

Objective:To observe the effect of huanglianwendan decoction on IR and leptin level of diet induced metabolic syndrome rat,and discuss the molecular mechanism that MS prevented and treated with huanglianwendan decoction.Methods:Sixty 8-week-old male SD rats were randomly divided into 2 groups which are vacuity group (n= 10) and experimental group (n=50). vacuity group was fed with common diet, and Experimental group was fed with high lipid, high salt and high glucose diet for 20 weeks to induce experimental metabolic syndrome.37 SD rats in experimental group which were modeled successfully were randomly divided into 4 groups:model group, huanglianwendan decoction high-dose group,low-dose group and Metformin Hydrochioride control group(n=8),which were lavaged with the appropriate drug for 4 weeks. Dynamic state measure weight, blood pressure, fasting blood glucose, serum insulin, lipids and other indicators, calculate HOMA-IR,to evaluate model and drug effect.Before and after drug therapy,we observed the level of leptin.Results:1.After feeding for 20 weeks, the weight, abdominal circumference, Lee's index, blood pressure, fasting glucose, fasting insulin, HOMA-IR, lipids TG, TC and LDL of Experimental rats were significantly higher, HDL level of reduction,compared with the vacuity group, the difference was significant (P<0.01).2.After 4 weeks treated with huanglian wendan Decoction, the weight and blood pressure decreased in Huanglian Wendan Decoction high and low-dose group and the metformin group, compared with the model group, in which Huanglian Wendan Decoction high-dose group and metformin group weight losssignificantly (P<0.01).3.The insulin resistance index(HOMA-IR) in Huanglian Wendan Decoction high-dose group and the metformin group decreased, Compared with model group, there was significantly different (P<0.01) Serum leptin concentration in MS model group increased, Compared with the vacuity group, the difference was significant (P<0.01); compared with the model group, serum leptin, concentrations of Huanglian Wendan Decoction high-dose group and metformin group rats decreased, the difference was significant (P<0.01);Conclusion:1. Results:high fat high sugar highsalt diet feeding SD rats for Long term can copy the MS model with the basic human clinical features of MS.2. Huanglian Wendan Decoction can loss weight, lower blood pressure, regulate lipid and improve insulin resistance, which can improve the various components of MS. Huanglian Wendan Decoction is an effective drug on preventing and treating MS.3. Huanglian Wendan Decoction can lower Serum leptin and improve insulin resistance,which may be one of the mechanisms to cure and prevent metabolic syndrome.