The Expression And Relevant Research Of IGF-Ⅱ,VEGF And MVD In Primary Hepatocellular Carcinoma

Objective:To explore the expression level of insulin-like growth factorⅡ(IGF-Ⅱ), vascular endothelial growth factor (VEGF) and CD34 antibody to tumor microvessel density (MVD) and their relevance in primary hepatocellular carcinoma (HCC) in guangxi.Method:The expression of IGF-Ⅱ, VEGF and CD34 were detected by immunohistochemistry in 50 cases of HCC in patients with resected hepatic cancer and adjacent tissues. Respectively, the intensity of IGF-Ⅱand VEGF expression were measured in the integrated optical density (IOD), CD34-positive organizations was count with MVD, and clinical data were statistically analyzed. Over the same period, 10 cases of liver transplantation in patients with resected liver tissues were as a normal control group.Results: IOD of IGF-Ⅱin HCC hepatic cancer tissue, adjacent tissue and normal liver tissue were 55940.00±44570.41,83688.81±64460.15 and 0; IOD of VEGF in hepatic cancer tissue, adjacent tissue and normal tissue were 37315.79±27315.09, 36181.70±27912.17 and 2528.96±1445.21. Ddjacent tissue, hepatic cancer tissue compared with normal tissue, IOD of IGF-Ⅱand VEGF were significantly different (P<0.05); the hepatic cancer tissue compared with adjacent tissue, IOD of IGF-Ⅱwas significantly different(P<0.05), IOD of VEGF was not statistically significant different(P>0.05). MVD in hepatic cancer tissue was 42.10±20.63, MVD in adjacent tissue was 28.96±8.55, MVD in normal liver tissue was 17.70±9.67, three were significantly differen(tP<0.01). Expression of IGF-Ⅱwas correlated with tumor size, HBsAg and histological differentiation degree(P<0.05), but not related with gender, age, metastasis of lymph node or liver, tumor thrombus, capsular and AFP(P>0.05).Live cancer tissue with high histological differentiation degree, little tumor and masculine expression of HBsAg, the expression level of IGF-Ⅱstrengthened, otherwise, weakened. VEGF and MVD were correlated with histological differentiation degree and metastasis of lymph node or liver(P<0.05), but not related with other factors (P>0.05). Live cancer tissue with high histological differentiation degree, metastasis of lymph node or liver, the expression level of VEGF and MVD strengthened, otherwise, weakened. MVD in positive expression of IGF-Ⅱand VEGF were 44.24±21.22, higher than MVD in single positive expression of IGF-Ⅱand VEGF(28.96±8.55)and MVD in negative expression of IGF-Ⅱand VEGF(19.25±14.45), the difference was statistically significant (P<0.05). IGF-Ⅱexpression in hepatic cancer tissue was significant positively correlated with VEGF (P <0.05). Conclusions: Expression of IGF-Ⅱin HCC was increased and maybe induce overexpression of VEGF, which was closely related with the aggressive biological behavior of HCC. IGF-Ⅱ, VEGF and MVD were positively related, suggesting that expression of IGF-Ⅱand VEGF may be involved in the regulation of tumor angiogenesis and have a synergistic effect.