Study On The HPLC Fingerprint And Efficacy Survey Of Chinese Herbs Astragalusfrom Different Parts Of Shanxi Province

Astragalus main chemical compositions include saponins class, flavonoids and polysaccharides etc. The 2005 version of China Pharmacopoeia only stipulate the astragalus armour。Recently,there are many research on Astragalus.The literature mainly reported the Astragalus armour glucoside, calycosin separately, the awn polypropylene content determination of flower element method and Astragalus medicine fingerprint research methods. Chromatographic fingerprint can all-roundly detect its composition in medical herbs and make the inner quality be visible of visual. Currently astragalus fingerprint mainly have HPLC, molecular biology method, etc.ObjectiveEstablish different HPLC fingerprint for Radix Astragalus from different parts of Shanxi Province; Provide references for scientifically evaluating the inherent quality of Astragalus.We send questionnaires in several hospitals in Shanxi,in order to verify effect of four origin Astragalus medicine on patients, so that a better future for astragalus medicine for guidance.MethodsHPLC, acetonitrile-water (30:70) binary gradient elution; flow rate of 0.8 mL/min; detection wavelength 208nm; column temperature:30℃.Students of Shanxi TCM academy 06 class ZhongYi Department send and complete the questionnaire survey, survey data use chi-square test statistical methods.ResultsThe ingredients of Astragaloside from different parts of Shanxi Province were well separated. According to the fingerprint, the quality differences of Radix Astragali from each origin were revealed.According to different origin of Astragalus in Shanxi Province as the main medicinal materials medicine after different patients survey, to perform a chi-square test, and sum rules, can show each origin astragalus herbs treatment of various diseases of the advantages and disadvantages, so as to make better reference of Astragalus.Conclusion1,According HPLC fingerprint for Astragalus gathered from 4 different producing areas in ShanXi Provice, Astragalus,grown in Hun-yuan,contains the largest quantity of Astragaloside,and thus should have priority in clinical perscription and reseach esperiment.2,According HPLC fingerprint for Astragalus produced in the same areas but different producing time,the quantity of Astragaloside presents an increase first followed by decrease,and thus the best time for collection should be in the third year or the the fourth year after plantation.3,According to the questionaire and statistics, Astragalus produed in Hun-yuan ranks the first for clinical prescription and those produed in Fan-shi comesthe next.