Expressions Of MMP-9 And PCNA In Telomerase And Alternative Lengtheining Of Telomere Osteosarconma And Thier Clinical Significance

Objective:To study the expressions of Matrix meta lloprot einas es-9(MMP-9) and proliferating cell nuclear antigen(PCNA) in telomerase and alternative lengthening of telomere osteosarcoma,and discuss the effects of M MP-9 and PCNA in the occurrence and development of osteosarcoma.Methods:The expressions of MMP-9, PCNA, human telomerase reverse transcriptasc(hTERT) and promyelocytic leukemia body(PML) were examined using immunohistochemical SP staining method in 27 cases of osteosarcoma and 10 cases of normal bone tissues.Results:â‘ The positive rates of MMP-9 and PCNA expressiong in osteosarcoma tissues were 88.89% and 85.19% respectively, in normal bone tissues were 10% and 0%,there were significant differences between two groups(P<0.001);â‘¡The expressions of MMP-9 in osteosarcoma of telomerase were twelve cases of (+++),five cases of (++),one case of (+),two case of(-).The expressions of MMP-9 in osteosarcoma of alternative lengthening of telomere(ALT) were zero cases of (+++),four cases of (++),two case of (+),one case of (-).there were statistically significant differences between telomerase and telomere alternatives osteosarcoma(P<0.05)..â‘¢The expressions of PCNA in osteosarcoma of telomerase were ten cases of (+++),six cases of (++),one case of (+),two case of(-).The expressions of PCNA in osteosarcoma of ALT were zero cases of (+++),four cases of (++),two case of (+),one case of (-).there were statistically significant differences between telomerase and telomere alternatives osteosarcoma(P <0.05). Conclusion:there were statistically significant differences of the expr essions of MMP-9 and PCNA between osteosarcoma of telomerase and telomere alternatives.The abilities of metastasis and invasion and the proliferation in telomerase osteosarcoma are stronger than alternative lengthening of telomere.