The Clear Lung And Blood Stasis Soup By Eliminating Phlegm AECOPD Rat Serum IL-4 And MMP-9 The Influence Of Expression

【objective】: To explore the phlegm stasis soup by clear lung to AECOPD rat serum IL-4 and MMP-9 the influence of expression.【methods】:Will clean level 60 female SD rats, only randomly divided into the blank group, the model group, the clear lung and stomach soup by eliminating phlegm to fill with penicillin muscle note large, medium and small dose group and the control group (penicillin flesh notes every group of each 10 only). Feeding adaptability, except the blank group a week rats are set on homemade airtight smoke exposure box, to 10 cigarettes every day, passive smoking 40 min for passive smoking 5 weeks. The 34 days, 10% of the first intraperitoneal injection of aldehydes (0.4 ml/chlorine 100 g) anesthesia to blank, rats, the endotracheal into 0.9% sodium chloride solution (0.01 ml / 100 g), to the rest of the group rats injected in the trachea porcine pancreatic elastase solution (25 U / 100 g). Continue to normal feed to 6 weeks from each group in a rat, take lung tissue with 10%, formaldehyde solution is fixed and the shaanxi institute of traditional Chinese medicine, send pathology staff room for pathology, judge success made mode. Give except blank rats, irrigation system of radix aconiti serve decoction 2 ml / 100 g/d, even served three days, induce rats appeared COPD acute exacerbation period. Make die, after the completion of blank group and the model group is assigned a 0.9% saline (3 ml / 100 g/d), divided into 2 times a day filling the stomach; Control group give penicillin injection (13.3 ku / 100 g/d, equivalent to the amount of adult 7 times), once a day; Large, medium, small dose group were injected penicillin, amount to with control group, and were given to clear lung by eliminating phlegm stasis soup decoction of (4.0 ml / 100 g/d, equivalent to 8.5 times the amount of adult); (3.0 ml / 100 g/d, equivalent to the amount of adult 6.25 times) and (2.0 ml / 100 g/d, equivalent to the amount of adult 4 times), divided into 2 times a day filling the stomach; Drug use time for continuous 14 days. Application elisa kit before and after treatment were level of serum MMP-9 and IL-4 change.【results】: large and medium-sized dose rats, serum IL-4, MMP-9 levels decreased significantly, in before and after treatment (P < 0.01); Large and medium-sized and small dose group of dose group and control group rat serum IL-4, MMP-9 level compared to a significant fall, (P < 0.01); Small dose group compared with the control group, (P > 0.05), no significant difference was statistically significant. Conclusion: the clear lung phlegm stasis soup by large, medium and small dose treatment, to cooperate with penicillin AECOPD rat serum IL-4, MMP-9 level are the influence of different level, of which the clear lung by ShangDaZhong dose with phlegm and blood stasis, penicillin therapy to significantly reduce AECOPD rat serum IL-4, MMP-9 levels.【conclusion】: Chinese medicine clear lung and stomach soup by eliminating phlegm to fill with western medicine treatment, can penicillin flesh notes significant reductions in AECOPD rat serum IL-4, MMP-9 levels.