Influence Of Tanshinone Ⅱa On Cellproliferation And Vascular Endothelial Growth Factor Expression In Human Bile Duct Carcinoma Cell Line HCCC-9810

ObjectiveTo observe the influence of TanshinoneⅡA on cell proliferation and vascular endothelial growth factor expression in human bile duct carcinoma cell line HCCC-9810 , and to explore its possible anti-tumor mechanism of action.MethodsHuman bile duct carcinoma HCCC-9810 cells were cultured in vitro and treated with tanshinoneⅡA. The cell proliferation was examined by the method of MTT; the transcriptions of VEGF mRNA were demonstrated by RT-PCR technique; Western blot was applied to examine the protein expression of VEGF.ResultsLow-dose(0.1mg/L)tanshinoneⅡA has no significant inhibitory effect on cellular proliferation of HCCC-9810 cells .When the concentration exceeded 0.25mg/L, tanshinoneⅡA significantly inhibited cellular proliferation in a time-and concentration-dependent manner, and down–regulated the mRNA and protein expression of VEGF (P <0.01).ConclusionWithin a certain drug concentration, tanshinoneⅡA can inhibit the proliferation of HCCC-9810 cells and play a potential role in inhibiting angiogenesis by down-regulating the expression of VEGF.