| Objective to confirm the chemical constituents in anti-cerebral ischemia fraction from Ixeris sonchifolia(Bge.) Hance,establish a reasonable quality control system,optimize the preparation process of the effective fraction and study their capacity of passing the blood brain barrier of SD rats.Methods 1.With the fingerprints of effective fraction,we isolated the major chemical constituents and identified their chemical structures.2.By measuring RCF,we established a method,which the content of all five compounds could be determined with one reference substance3.From extraction, alcohol precipitation and column chromatography, three aspects of preparation process was optimized.4.SD rats received intraperitoneal injection of the effective fraction and the major constituents.The different HPLC of cerebrospinal fluid was compared.Results l.Got five major constituents from the effective fraction:caffeic acid(Is-1),cichoric acid(Is-2),luteolin-7-0-β-D-glucopyranoside(1→6)-β-D-glucopyranoside(Is-3), luteolin-7-O-β-D-glucuronopyranoside(Is-4),apigenin-7-O-β-D-glucuronopyranoside (Is-5).the content of five components was around 96% in fingerprints.2.The relative correction factors of caffeic acid, cichoric acid,luteolin-7-O-β-D-glucopyranoside(1→6)-β-D-glucopyranoside, apigenin-7-O-β-D-glucuronopyranoside were identified as 1.129,0.8733,1.320,1.1293.This method was almost no different with external standard method.4.The total content of all the five major constituents in the effective fraction was around 65% and the content of major categories was around 84%.4.No parent drug was found in the cerebrospinal fluid, but some metabolin were found in CSF in Is-2 group.Conclusions Confirmed the chemical constituents in effective fraction,established a reasonable quality control system,got effective fraction which met the requirement of TCMI,study on passing BBB laid a foundation for further study on mechanism of anti-cerebral ischemia.
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