Research On Preparation Process, Quality Standards Of Dipsacus Sapogenin And Its Inhibitory Effect On Alpha-glucosidase

Studies on the preparation procedures, Quality Standard and Inhibition Activity to (?)-Saccharase of Dipsacus Asper SapogeninIn this paper, the prepakation procedure, guality standard and inhibition activity to (?)-saccharase of dipsacus asper sapogenin have Been researched. The obtained results would become a foundation for developing safe and effective lowering blood sugar drug.1. Studies on the extraction and purification procedures of dipsacus asper saponinUsing dipsacus asper herb as the raw material, the extraction procedure of dipsacus asper saponin was aptimized by orthogonal design experimentes with the extrational yeild of hederagenin, an essential component in dipsacus asper herb, as the evaluation index. The optimized procedure was as following:The herb raw material was refluxed to extract for 3 times with 80 wit% ethanol, the extraction period of each time was 3 hurs,2 hurs and 1.5 hurs,respectively.Used volue (L) of 80% ethanol to the herb weight (kg) for every time extraction was 6 times,5 times and 5 times, respectively.Then obtained such rough extractat was purified through a marcro porous resin column and eluted by water and 70 wit% ethanol subsequently, then obtained ethanol eluetion solution was concented and dried, the resedue was abtained as the total dipsacus asper sapanin2. Studies on hydrolis and purification process of dipsacus asper saponinThrough appling an orthogonal experiment and using hederagenin yeild as the evaluation index, an optimized hydrolis procedure was established. The process is as following: Former obtained saponin (kg) is added into a 15 time volue solution of 2 mol,L-1 HCL solution of 40% ethanol and is hydrolysed for 3 hurs,then the hydrolytate was heated to boil and is dissolved in ethanal of 15 time volue, then the pharmaceutical active carbon of 1% weight is added into it and is heated again to boil for certen time, more then it is filtered and concentrated and ried, the residue is the product sapogenin of dipsacus asper saponin.3. Separation and dentification of the main components in obtained dipsacus asper sapogeninFormer obtained sapogenin was separated by using a C180DS column at first, then, seperated product was charactrizated and identified by melting point measurement, IR spectra UV-Vis spectra, NMR and MS analysis. The results showed that the main components in the product was hederagenin an enssential component in dipsacus asper, and a lesser component was oleanolic acid.4. Establishment of the guality standard of dipsacus asper sapogeninThe guality standard of sapogenin product was determed using differement analysis methods.The hederagenin in product was identified with TL method The dry less weight of the product was determed to be no over 5 wit%. The ignition residue was determed to be no over 0.5%. The content of arsenate and weight matels were determed to ought to be agree with pharmaceutia standard. The content of total sapogenin in the product was determed to be 90% to 110% by using UV-vis spectro photometer method.The content of hederagenin in the product was determed to be 70% with HPLC method.5. Effect of the dipsacus asper sapogenin on (?)-SaccharaseThe results of saccharose hydrolys test and the saccharase actity test of intestines turn of animal in vitro showed that the dipsacus asper sapogenin possesses a quite better in hibition activity to (?)-Saccharase, and the effected target enzyme were determined to be the saccharidases and amylase.