Determination And Technical Study On Extraction Separation Of Hypericin Form Hypericum Attenuatum Choisy

The subject researched the extraction-purification technology of hypericin in Hypericum attenuatum Choisy, and established a method of determination of hypericin in Hypericum attenuatum Choisy.In extraction technology process,We investigated the influence of four factors:density of ethanol,the quantity of extraction solvent, extraction time, extraction number of times to extraction technology.In the end, we determined the optimum extraction technology of the hypericin in Hypericum attenuatum Choisy is:70% ethanol as solvent, the ratio of material and solvent is 1:8, extracting three times with 1h for each time at 80℃In purification technology process, through sieving, we use macroporous adsorption resin to purify the hypericin in Hypericum attenuatum Choisy. We investigated the influence of factors:Macroporous resin models,Resin column "diameter high-ratio" concentration of the sample extract, the sample rate, adsorption time, elution flow rate, elution, resin regeneration time. In the end, we determined the optimum purification technology of total flavonoids in Speranskia tuberculata is:take the sample by 2BV/h for 2 times, after adsorbing 2h, eluting with H2O and 40% ethanol, at last use 80% ethanol and ethanol recovery.After the extraction and purification method, crude product obtained by hypericin content was 10.6mg·g-1 (hypericin content in Radix 0.5 mg·g-1), significantly improved the purity of hypericin for the next step in the development and utilization of hypericin has laid a good foundation. In order to better carry out research and development of Ukraine and the use of Hypericum gland, norm-setting, scientific method for the determination. To index the content of hypericin, chromatographic conditions were column Aglient SB C18 (4.6x250mm.5μm), mobile phase was methanol-pH6.5 phosphate buffer (90:10), flow rate of 1mL/min The column temperature was at room temperature, detection wavelength 590nm, results show that the determination of linearity, precision, stability, good reproducibility and recovery.