| Objective:to establish behavior despair model and chronic stress, depression model mice to Hypericum attenuatum Choisy. Observe the depression model, and the influence in mice behavior of Hypericum attenuatum Choisy. Chronic stress depression model, this paper discusses the influence of the antidepressant effects and effective parts.Methods:1. Behavior despair tail suspension, model used mice forced swimming experiment method, mouse randomly divided into blank group and Hypericum attenuatum Choisy. Water extraction low doses groups and Hypericum attenuatum Choisy. Dose group of uzziel and water improve Hypericum attenuatum Choisy. Ethanol-extraction low dose group and urumqi-kuitun Hypericum attenuatum Choisy alcohol increase dosage group, positive medicine 100 sorrow solutions group. 7 days after the test group mice tail sus-pension and swimming fixed time, and opening experiment.2. Chronic stress mice mouse randomly divided into depression model control group (not inflict any stimulus), chronic mild can not foresight stress depression model group and urumqi-kuitunHypericum attenuatum Choisy extraction low doses groups and urumqi-kuitun Hypericum attenuatum Choisy dose group of uzziel and water improve Hypericum attenuatum Choisy ethanol-extraction low dose group and urumqi-kuitun Hypericum attenuatum Choisy alcohol increase dosage group, positive medicine 100 sorrow solutions group, in addition to control group outside, the rest are and accept every group rat stressor stimulation, including fast, the forbidden 24h,40 degrees heat water ice 5min,4 degrees 7min, electrocution 5min, swimming, day and night reverse 5min clips tail 24h all 7 kinds of stimulating randomly assigned to 21 days, daily 1 species, each stimulate appear three times, the same stimulation can't use continuously, to prevent the occurrence of animal expect stimulation. 21 days after taking brain, measurement of bufo single amine oxidase, superoxide dismutase (sod) and of mda.Results:1. In the tail suspension and forced swimming trials, compared with control group, irrigation dosing 7 days, gastric adenocarcinoma water Hypericum, low water high doses can significantly reduce the mice group were fixed time (p<0.05), and uri Hypericum attenuatum Choisy alcohol high-dosage groups, low alcohol can significantly reduce the mice are fixed time (p<0.01). In chronic stress, depression model experiments in mice dosing filling,7 days stomach in treatment group and the blank group had no significant difference compared (p>0.05).2 In chronic stress,depression model experiments in mice 21 days, build mode group compared with model built both groups was statistically significant, including water, low water high group has significant difference (p<0.05), alcohol, low alcohol there are very significant differences high group (p<0.01); SOD alcohol was significantly high group differences (p<0.001), MDA alcohol there were significant differences in high group (p<0.05).Conclusion:1. The Hypericum attenuatum Choisy can reduce udinese behavior of the fixed despair model mice antidepressants, have time to ethanol-extraction group effect is good.2. Water and ethanol-extraction content can reduce depression within the content of small brains built, including water and alcohol improve dose increase dosage particularly significant effect. Alcohol improve dose could decrease the depression in the content of MDA small brains, water mention ethanol-extraction that they can increase depression small brains, the content of which in SOD alcohol improve dose particularly significant effect.3. Ethanol-extraction contains its antidepressant effective parts. Ethanol-extraction low and high dose group effect quite. |
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