H.Pylori Infection And Peripheral Blood Lymphocyte Subsets From Family Members Of Children With Recurrent Abdominal Pain

Objective:To investigate the prevalence and the phenotype of Helicobacter pylori (Hp) infection in family members of children with recurrent abdominal pain. To study the peripheral blood Lymphocyte subsets in family members of children with recurrent abdominal pain, define immune function conditions, to explore immunological mechanisms of the Helicobacter pylori-related gastrointestinal diseases.Methods:Hp immunophenotyping method was used to detect the Hp type of 50 family members and 55 volunteers, made the Hp negative ones to be control. Venous EDTA blood specimen was obtained for Lymphocyte subsets analysis by flow cytometry. Divide those people in two ways: divide into recurrent abdominal pain group and the normal control group according to symptoms; divide into children with recurrent abdominal pain, family members with recurrent abdominal pain and the normal control group according to symptoms and ages.Results:1. The results of Hp immunophenotyping method:the prevalence of Hp I type is 90%,2.0% is Hp typeⅡ,4.0% is negative in all the 50 family members.25 ones are Hp I type and 30 are negative among 55 volunteers. There is no significant difference between children and elders group about the positive rate of Hp infection of 50 family members in gender and age level(X2=0.007, P=0.933; X2=1.259, P=0.533);There is no significant difference between children and elders group about the prevalence of HpⅠ,Ⅱtype of 50 family members in gender and age level (X2=1.115, P=0.291; X2=1.453, P=0.484).2. The results of lymphocyte subsets:Peripheral blood lymphocyte subsets in each group CD3+(%),CD19+(%),CD4+(%),CD8+(%),CD16+56(%),CD4+/CD8+ as follows:recurrent abdominal pain group: 65.74±7.95,3.48±5.21,34.48±7.09,26.48±6.24,19.33±7.54,1.38±0.46; children with recurrent abdominal pain:65.45±8.37,16.55±5.89,32.09±4.85,27.64±5.77,16.18±6.68,1.20±0.26; adult with recurrent abdominal pain:65.83±7.94,12.51±4.65,35.23±7.56,26.11±6.41,20.31±7.61,1.43±0.50; normal control group:69.09±7.23,12.29±9.67,34.29±6.12,31.68±6.69,19.38±7.32,1.16±0.35. Compared with the normal control group, CD19+(%) in recurrent abdominal pain group was increased(X2=4.344,p=0.037), and in children with recurrent abdominal pain was increased when compared with the other two groups (X2=8.989,p=0.011); Compared with the normal control group, CD8+(%) in recurrent abdominal pain group was significantly reduced (t=-3.573,p=0.001), so was in children group and adult group with recurrent abdominal pain (F=6.574, p=0.002), but no significantly different between those two groups; Compared with the normal control group, CD4+/CD8+ in recurrent abdominal pain group was increased (F=4.109, p=0.020), Compared with children with recurrent abdominal pain and normal control group, CD4+/CD8+ in adult recurrent abdominal pain group was increased(F=4.109,p=0.020); there was no significantly different about CD3+(%), CD4+(%), and CD16+56(%) in other groups and three groups.Conclusion:There is no significant difference about the positive rate of Hp infection and the prevalence of HpⅠandⅡtype of family members in gender and age level. There is familial cluster phenomenon in family members suffering from recurred abdominal pain. There was cellular immunity disorder in family infection with Helicobacter pylori, B cells and T cell immune response were adaptive immune response. The phenomenon may be related with the development and outcome of helicobacter pylori-induced gastrointestinal diseases. Further research is required.