Helicobacter Pylori Infection And HLA-DRB1﹑DQA1﹑DQB1Allelic Frequencies In The Families Of Children With Recurrent Abdominal Pain

OBJECTIVE:To investigate the prevalence of Helicobacter pylori (Hp) infection and the strains of Helicobacter pylori in the family of children with recurred abdominal pain. To explore human leukocyte antigen DRB1,DQA1,DQB1allele frequencies in these family. To investigate the relationship between Hp infection and human leukocyte antigen DRB1,DQA1,DQB1alleles, and find the correlative human leukocyte antigen after Hp infection in the families with and without recurrent abdominal pain, so that we can find if there are some associated genes with recurrent abdominal pain after Hp infection.METHODS:The Hp immunophenotyping is determined by Western blot (immunoblot) technique among196members in41families of children with recurred abdominal pain. All members were divided into with Hp positive and Hp negative group. And Hp-positive members are divided into recurred abdominal pain group and without recurred abdominal pain group. PCR-SSP technique is applied to identify HLA-DRB1,DQA1,DQB1allelic frequencies.RESULTS:The Hp subtype antibody positive rate in the Western blot is87.2%. The prevalence of Hp I typeis57.7%.The prevalence of Hp type II is29.6%. There is significant difference between children and elders group about Hp strains rate (P<O.05). HLA-DRB1*1204,*14,*1527,1534allelic frequency is lower in the members with Hp infection than that in the members without Hp infection,(0%vs6%,x2=20.839, P=0.000, Pc<0.05;6%vs20%,x2=12.587, P=0.000, Pc<0.05;1%vs8%,x2=16.166, P=0.000, Pc<0.05). HLA-DQAl*040101,*040102allelic frequency is lower in the members with Hp infection than that in the members without Hp infection,(1%vs9%,x2=11.791, P=0.001, Pc<0.05;0%vs9%,x2=18.032, P=0.000, Pc<0.05). HLA-DRB1*09allelic frequency is higher in the members after Hp infection with recurred abdominal pain than that in the members after Hp infection without recurred abdominal pain, but the signification all disappeared when the allelic Bonferroni correction for comparisons were applied as required (12%vs3%,x2=8.555, P=0.003, Pc=0.054>0.05). HLA-DQAl*0302allelic frequency is significantly higher in the members after Hp infection with symptoms of recurrent abdominal pain than in the members after Hp infection without symptoms,(14%vs3%, x2=11.272, P=0.001, Pc<0.05)CONCLUSIONS:The seropositive rate of Hp antibodies is high in the family with recurred abdominal pain. There is familial cluster phenomenon in family members suffering from recurred abdominal pain. There is significant difference between children and elders group about Hp strains rate, increased with age Hp infection rate tended to grow. There are HLA-DRB1*1204,*14,*1527,1534and DQA1*040101,*040102allelic frequency difference between the members with Hp infection than that in the members without Hp infection. HLA-DRB1*1204,*14,*1527,1534and DQA1*040101,*040102may be a correlative gene to protect people from Hp infection. It should be studied further whether HLA-DRB1*09allele are associated with abdominal pain. HLA-DQAl*0302may be an associated gene contributed to symptoms of recurrent abdominal pain after Hp infection.