Study On The Establishment Of Hairy Roots Culture System And Its Secondary Metabolites Of Abelmoschus Esculentus L.

Abelmoschus esculentus L, which belongs to Mallow family of Annual herb, originating in Africa tropical regions. Its Flower, seeds, root all can be used to heal, has good curative on carbuncle and malignant sore. Besides, Abelmoschus esculentus L be rich in multi vitamin, trace elements, protein, fattiness, soluble fibre, polysaccharose, flavone, alkaloid and so on. Usually eat can help digest, reduce blood fat,boost up hominine resistance and physical force. Not only intestines and stomach, liver, skin and mucosa can be protect by Abelmoschus esculentus L,but also can cure gastritis, gastric ulcer, illness of liver. Abelmoschus esculentus L by the name of "Plant Viagra", Because it has many effective medicinal ingredients that has better on enhance physical endurance and strong nephridium.The result show that, R1601 is the best agrobacterium rhizogenes for infected by Abelmoschus esculentus L. When place the leaf face up on the culture medium, the infection time is 8 min, the optimum pre-culture time is 2d～3d, exhibits the highest infection hairy roots.The PCR detection results of hairy roots show a strap that between 500～750bp as the rolC gene. Indicate that T-DNA of Agrobacterium Ri Plasmid has already integrated into hairy roots' genome.The transformed hairy roots in the culture medium rapid growth whitout hormone that showed typical character:dense hairy roots, much branch and grew upwards. The hairy roots of Abelmoschus esculentus L were induced by effects of differrent basic media, different sucrose concentration and exgenous phytohormones on growth influenced. Study proves that:MS is the best media on increment, after 25d cultivated in culture medium which hairy roots increase 26 times, the worst is White that hypohaline culture medium, just increase 3 times; increment multiple get a tiptop when the sucrose concentration is 3%, accord with literature report; and the optimum hormone for the hairy roots is 0.5mg/L IAA, the increment multiple is 48.55 times,1.87 times highter than cultivated without hormone.Separately extract from Abelmoschus esculentus L hairy roots get flavone and polysaccharide, determine their content in hairy roots used Phenol-sulfuric acid method and aluminum nitrate development process. Study proves that the flavone content is 16.4%,5.9 times highter than plant. The polysaccharide content is 16.6%,10.11 times highter than plant.Flavone is a strong antioxidant, can clean oxygen free radical in body, and removal bacteria. During the research, the flavone of the hairy roots have obvious antibacterial activity for Escherichia coli ATCC8099,50 mg/ml extraction's percentage of bacteriostasis is 86.67%,highter than 25mg/ml extraction's 66.67%, the rate of the 12.5mg/ml extraction is 0. But no any bactriostasis for the Staphyloccocus aureus Rosenbach ATCC6538.