Chemical Constituents From Abelmoschus Esculentus Linn Moench And Euphorbia Hirta Linn

This dissertation focused on the systematic investigation of the compounds from the Abelmoschus esculentus Linn Moench and Euphorbia hirta Linn., through the technological methods common in modern natural products chemistry.From the two plants, a total of18compounds were isolated through column chromatrographies over silica gel, Sephadex LH-20, MCI, preparative TLC and preparative HPLC etc. Through extensive analyses of the spectroscopic data including1H-NMR,13C-NMR and ESI-MS, their structures were identified as hibiscolactone A (1), boehmenan (2), boehmenan X (3),4-O-methylcedrusin (4), p-Hydroxyphenethyl trans-ferulate (5),2-(4-hydroxy-3-methoxy phenyl) ethyl-3(4-hydroxy-3-methoxy phenyl)-acry late (6), carolignan (7), dimorphamide A (8), N-trans-coumaroyltyramine (9), N-trans-feruloyltyramine (10), trans-feruloydopamine (11), Arijunlic acid (12),2β,16α-dihydroxy-ent-kaurane (13),16a,19-dihydroxy-ent-kaurane (14),(-)-clovane-2,9-diol (15), hexa-O-methylmyricetin (16),5,7-dihydroxy-3-methoxy-2-(4-methoxy-phenyl)-chromen-4-one (17), quercitrin (18). All of them were known compounds, including five lignans(2,3,4,7,8), three amides(9,10,11), one oleanane-type triterpene(12), two ent-kaurane diterpenes(13,14), one sesquiterpene(15), three flavones (16,17,18) and three others. The12compounds from Abelmoschus esculentus Linn Moench and the compound15from Euphorbia hirta Linn were isolated for the first time from the plants. The structures of them were shown as following: The compounds carolignan2(boehmenan),5(p-Hydroxyphenethyl trans-ferulate),7(carolignan),8(dimorphamide A) and some fatty acids, were applied for the preliminary evaluation of their antitumor activities against human cervical tumor cells Hela and Caski by SRB assaies, respectively. The results were shown that compound7showed inhibition activity against Hela and Caski with IC50valves below10μM.In summary, through a systematic investigation on the chemical constitutes of the two plants, eighteen compounds were isolated and structurally elucidated. The preliminary evalution showed that compound7exhibited inhibitory activity against cervical tumor cells. Our finding enlarged the knowledge of the chemical base of these herbs and provided more references for its experiment and medicinal usage research.