Fgl2 Expression In The Microcirculation Dysfunction Of The Myocardial Ischemia/Reperfusion Rat Model

Part I Microcirculation dysfunction in acute ischemia/reperfusion ratsObjective: To establish a rat model of myocardial ischemia/ reperfusion . To investigate the myocardium no-reflow and heart function of this model and the role of microthrombosis in the myocardium no-reflow and heart function change of this model.Methods: Sixty male SD rats were randomly divided into normal control group (NC, n=12), sham-operated group (sham, n=12),operation-treated group (n=36). The operation-treated group were randomly divided into 1 days group (1d, n=12),3 days group (3d,n=12), 7 days group (7d, n=12) by the reperfusion time . To observe the heart function of rats by hemodynamics and echocardiography at the end of the reperfusion. To observe the areas of no-reflow and myocardium necrosis by TTC and thioflavine S decoration. To dissociate the PBMC from the blood of the rats, and store in liquid nitrogen。To select part of ischemic myocardium store in liquid nitrogen. To detect microthrombosis in the rest of ischemic myocardium by HE and MSB staining.Results: 1. Echocardiography shows abnormal movement of left ventricular wall in myocardial ischemia/ reperfusion rats. Hemodynamics shows -dp/dtmax reduction in myocardial ischemia/ reperfusion rats. 2. Part of the ischemia myocardium has no restoration of blood flow and exists myocardium necrosis. 3. HE staining: Myocardium is regular in the group of NC and sham, myocardium necrosis can be seen in myocardium ischemia/ reperfusion rats. 4. MSB staining: Microthrombus exists in myocardium microvasculature of ischemia/ reperfusion rats. There are more microthrombus in 3d group than 1d group and 7d group.Conclusions: Microcirculation dysfunction exists in the myocardial ischemia/ reperfusion model and results in the reduction of the Left Ventricular Diastolic Function of this model. Microthrombosis may play an important part in the microcirculation dysfunction of myocardium.PartⅡFgl2 expression of myocardium and PBMC in ischemia/ reperfusion rats and correlation.Objective: To investigate the expression of fgl2 in myocardium and PBMC of this model. To analyse the relationship between expression of fgl2 in PBMC and myocardium.Methods: To detect the expression of fgl2 in myocardium by immunohistochemistry Sabc methods. To detect the expression of fgl2 in myocardium and PBMC by Western blotting.Results: 1. Immunohistochemistry Sabc methods show expression of fgl2 in Microvasculature. 2. Western blotting shows expression of fgl2 in myocardium and PMBC increase in myocardium ischemia/reperfusion rats. 3d group has more fgl2 expression than 1d group and 7d group. The positive correlation exists between fgl2 expression of myocardium and PBMC.Conclusions: Fgl2 expression increases in myocardium and PBMC of the myocardial ischemia/reperfusion rat model. Fgl2 expression of microvasculature may play an important part in microthrombosis of myocardium. The positive correlation exists between fgl2 expression of myocardium and PBMC.