Studies On The Chemical Constituents Of Viburnum Foetidum Var. Ceanothoides And Viburnum Odoratissimum Var. Awabuki

Viburnum foetidum var. ceanothoides belongs to the genus Viburnum (Adoxaceae), distributed in Yunnan, Sichuan, Guizhou provinces of China. The roots, barks, leaves and fruits have been used in Chinese folk medicines to treat stomatitis, eczema, fractures, bone-setters injury and trauma hemorrhage.It hasn't been reported about the research of V. foetidum var. ceanothoides so far. We studied on the chemical constituents from the branches and leaves of V. foetidum var. ceanothoides to find bioactive compounds and promote its further research and usage.Viburnum odoratissimum var. awabuki belongs to the genus Viburnum (Adoxaceae), distributed in Zhejiang province, Taiwan Island, Japan and South Korea, and also planted by the Yangtze River. It's a kind of excellent landscape tree because of absorptive capacity for toxic gas. It's been used for hundreds of years as fish stupefying agent to catch fish in South Asia and Okinawa Prefecture. It hasn't been studied on the introduced V. odoratissimum var. awabuki in the lower Yangtze River of China. We took a preliminary study on the chemical constituents to investigate the difference between the original species and introduced ones and find new vibsane-type diterpenoids.The main contents of our study are as following three aspects:1. The chemical constituents of V. foetidum var. ceanothoides and V. awabuki were studied base on the physiochemical and spectroscopic evidences. Fourteen compounds were isolated from V. foetidum var. ceanothoides, including triterpenoids, phenols, sterols, flavonoids and so on. They are as follows: Betulin(VF-1), Uvaol(VF-2),β-Sitosterol(VF-3), Betulinic acid(VF-4), Ursolic acid(VF-5), p-Hydroxybenzoic acid(VF-6), 4,4'-Dihydroxy-a-truxillic acid(VF-7), E-p-Coumaric acid(VF-8), Z-p-Coumaric acid(VF-9), Phaseic acid(VF-10), Protocatechuic acid(VF-11), Daucosterol(VF-12), 1-O-(6-O-α-L-Rhamnopyranosyl-β-D- glucopyranosyl)-4-allylbenzene(VF-13), Apigenin 7-O-α-L-rhamnopyranosyl(1'''→2'')-β-D-glucopyranoside(VF-14). Among them, VF-1, VF-7, VF-9, VF-10 and VF-13 were characterized for the first time from the genus Viburnum. Five compounds were isolated from V. odoratissimum var. awabuki. They are as follows: a-Amyrin(VA-1), Ursolic acid(VA-2), 6α-Hydroxylup-20(29)-en-3-on-28- oic acid(VA-3), 2a,3β-Dihydroxyurs-12-en-28-oic acid(VA-4), Daucosterol(VA-5). Among them, VA-1, VA-4 and VA-5 were characterized for the first time from this plant.2. This dissertation studied on inhibitory activities against LPS-induced NO production in macrophages and cytotoxicity against tumor cells of the main compounds(VF-1, VF-2, VF-4, VF-7, VF-8, VF-10, VF-11, VF-13, VF-14) from V. foetidum var. ceanothoides, in order to elucidate its bioactive constituents and provide the basis for further development.In bioassay on LPS-induced NO production in RAW 264.7 macrophages, 9 compounds didn't show significant anti-inflammatory effect.In the cytotoxicity test with MTT method against 4 kinds of tumor cells (A549, BGC-823, K562, K111), all the compounds didn't show significant anti-proliferative activities.3. Based on two reviews (Chem. Biodivers. 2010, 7, 567-593 and Chem. Biodivers. 2008, 5, 1879-1899), a review of the constituents and biological activities about genus Viburnum was supplied, which including another more 150 compounds.In addition, we summarized spectral data of vibsane-type diterpenoids, which may promote genus Viburnum's research and usage.