Studies On The Chemical Constituents And Biological Activities From Petroleum Ether Extract Of Viburnum Fordiae Hance

Viburnum.a genus belonging to the family Adoxaceae(formerly Caprifoliaceae),consists of more than 230 species of shrubs or small trees.Viburnum fordiae Hance,widely distributed in China,belongs to the genus Viburnum.As a traditional Chinese medicine,it has been widely used for the treatment of rheumatoid arthritis,lymphadenitis,tumefaction,cough and infantile tantrum.32 compounds were isolated from V.fordiae Hance by using various chromatographic and recrystallized methods(silica gel,RP-C18,MCI GEL,Sephadex LH-20 column chromatography,PTLC and HPLC).Their structures including absolute configurations were determined by spectroscopic and chemical methods.Triterpenoids:uvaol(1),28-nor-urs-12-ene-3,β,17β-diol(2),3-O-isopropylidenyl-2α,3α,19α-trihydroxyurs-12-en-28-oic acid(3),erythrodiol(4),oleanolic acid(5),lupeol(6);monoterpenoids:megastigmadien-3,9-dione(7),loliolide(8),dehydrololiolide(9),2a-hydroxycineole(10);lignanoids:(+)-isolariciresinol(11),fordianole A(12),fordianole B(13),fordianole C(14);phenylpropanoids:umbelliferone(15),3-(4-hydroxy-3-methoxyphenyl)propane-1,2-diol(16),7,8-bis-O-isopropylidene-dihydroeugenol(17),4-(3,4-dihydroxyphenyl)-5-methyl-dihyro-furan-2-one(18),1-(4-hydroxy-3-methoxyphenyl)-1-methoxypropan-2-ol(19),coniferyl aldehyde(20),p-hydroxylcinnamaldehyde(21),(+)-2-hydroxy-1-(4-hydroxy-3-methoxypheny)propan-1-one(22);phenolic acids:syringaldehyde(23),protocatechuate(24),3,4-dihydroxybenzoic acid methyl ester(25),vanillin(26),p-hydroxybenzaldehyde(27),salicylic acid(28),hydroquinone(30);others:benzyl alcohol(29),1-methyl-cyclopentene(31),β-sitosterol(32).Among them,12-14,17 and 18 were new compounds,and compounds 2,3,7-11,16,19-29 and 31 were isolated from this genus for the first time.The anti-inflammatory and antioxidant activities of the three extracts(PE,EtOAc and n-Butanol)from V.fordiae and compounds 1-30,isolated from the PE extract,were evaluated in vitro.The PE partition had preferable anti-inflammatory and antioxidant activities.And 20,21,30 showed potent inhibitory activity on NO production in RAW264.7 cells stimulated by LPS.11-14,18,20,21,23,24,26,27,30 exhibited varying antioxidant activities through DPPH,ABTS free radical scavenging and FRAP assays.