Studies On The Active Contituents Of Viburnum Propinquum And Viburnum Erosum

The genus Viburnum (Caprifoliaceae) includes ca. 200 species, widely distributed in temperate zone in northern and hemisphere subtropical regions. About 74 species were widely distributed in China, especially in Southwest China. Several species are employed as folk medicines to treat different diseases, such as cough, diarrhea, rheumatoid, arthritis and tumefaction. Extensive studies of the genus Viburnum have led to the identification of many compounds, such as diterpenoids, triterpenoids, iridoids, monoterpenes, sesquiterpenes, flavonoids, and lignans, including a number of compounds with good antioxidant and anti-tumor activity.Viburnum propinquum Hemsl. is a kind of evergreen shrubs, distributed in Shanxi, Gansu, Zhejiang, Jiangxi, Fujian, Sichuan, Taiwan, Guizhou, Yunnan provinces of China. It has been used in Chinese folk medicines to treat Fractures, bone-setters injury, trauma hemorrhage. Viburnum erosum Thunb. is a kind of deciduous shrub, distributed in east China, Shanxi, Guangdong, Guangxi and Southwest of China. It has been used in Chinese folk medicines to treat stomatitis, eczema.In this dissertation, we investigate the chemical constituents from the branches and leaves of Viburnum propinquum Hemsl. Besides, some of compounds from the title plant have been evaluated their antioxidant activity. Fifteen compounds were isolated by Silica gel chromatography, Sephadex LH-20 chromatography, and all of the compounds were identified by spectral analyses as follow: VP-1: 3, 4, 2′, 4′-tetrahydroxy-trans-chalcone, VP -2: 3, 4, 2′, 4′-tetrahydroxy-trans-chalcone-2′-O-β-D-glucoside, VP -3: (7-αH, 8′-αH)-4, 4′, 8α, 9-tetrahydroxy-3, 3′-dimethoxy-7, 9′-epoxylignan, VP -4: Taraxerol, VP -5:β-sitosterol, VP-6: Stigmasterol, VP-7: 3β, 28-dihydroxy-12-ursene, VP-8: Ursolic Acid, VP-9: Daucosterol, VP-10: 4, 2′, 4′-trihydroxy-dihydrochalcone, VP-11: 4, 2′, 4′-trihydroxy-dihydrochalcone-2′-O-β-D-glucoside, VP-12: Quercetin, VP-13: Dihydroquercetin, VP-14: 7, 3′, 4′-trihydroxy-flavanone, VP-15: Eriodictyol. Among them, VP-2, VP-3 were new compounds, and VP-1, VP-4, VP-10, VP-11, VP-13,VP-14, VP-15 were isolated from Viburnum genus for the first time. We also investigate the chemical constituents from the branches and leaves of Viburnum erosum Thunb. Eight compounds were isolated by silica gel chromatography, Sephadex LH-20 chromatography and identified by spectral analyses as follow: VE-1:β-sitosterol, VE-2: Ursolic Acid, VE-3: 5, 3', 5′-trihydroxy-7-methoxy-dihydro-flavone, VE-4: Daucosterol, VE-5: (-)- lyoniresinol 3α-O-β-D-glucopyranoside, VE-6: (+)- lyoniresinol 3α-O-β-D-glucopyranoside, VE-7: Adoxoside, VE-8: Loganin. Some compounds from Viburnum propinquum Hemsl were evaluated for their antioxidant activity. Compounds VP-1, VP-2, VP-12, VP-13, VP-14 exhibited antioxidant activities in 1, 1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical, and lipid peroxidation assays.