Study On Extraction, Purification And Quality Analysis Of Anti-inflammatory Active Site Of Smilax China L.

Smilax china L. popularly known in China as "Jin Gang Teng", belongs to the Liliaceae family. It's commonly used Chinese medicine, which was collected in the "The Chinese Pharmacopoeia" in 2005 version. It has various pharmacological effects including relieve sickness wet and disintoxication and so on. In China it has been extensively used for clinical treatment of anti-inflammatory, anticancer and axtioxidant activity. The Chinese medicine preparation of the active sites of Smilax china L. has good therapeutie efficacy against annexitis, and various gynecological inflammation diseases.Using the extracted method of reflowing ethanol and D101 macroporous resin purification technology to extract, separate and purify the active site of Jin Gang Teng, which obtain a reliable technical parameters. Carried out the verifying will be a transition to industrialization.With Quercetin, diosgenin as reference substance, using TLC method to identify the extract of Smilax china L.; with rutin, dioscin, gallic acid as reference substance, using UV method to detect total flavonoids, total saponins, total tannin of the extract of Smilax china L.. The quality standard were improved which can provide a basis for quality control.It is the first that formulate the fingerprint of the extract of Smilax china L. by HPCE. The analytical method has good credibility and stability. According to country medicines and chemical reagents food without exception superintending the relevance request that the administration bureau promulgates, fourteen shared peaks were found in the fingerprints and four peaks was identified to Resveratrol,rutin,kaempferide,Quercetin. The method is stable, well repeated and may offer abundant scientific basis of the quality evaluation of the extract of Smilax china L..