The Relationship Between Single Nucleotide Polymorphism Of IFN-γ, IL-10 And The Susceptibility Of Hand, Foot And Mouth Disease

Objective:To explore the possible relationship between the level of IFN-γand IL-10 in blood plasma and hand, foot and mouth disease(HFMD) and investigate whether the single nucleotide polymorphism (SNP) of IFN-γ+874 T/A and IL-10-1082 G/A correlate with HFMD.Method:By reverse transcription polymerase chain reaction(RT-PCR), we made use of specific primers to detect enterovirus71(EV71) and coxsackievirus A16(CA16) in the faeces of HFMD children. The levels of IFN-y and IL-10 in blood plasma were detected by enzyme-linked immunosorbent assay. The genotypes of IFN-γgene+874 site A/T and IL-10 gene-1082 site G/A were detected by the PCR techniques in EV71 patients, CA16 patients and normal children.Result:①EV71 was detected positive in 82 specimens from 178 acute stage children with HFMD. The positive rate was 46.07%. CA16 was detected positive in 67 specimens from 178 acute stage children with HFMD. The positive rate was 37.64%.②Compared with the normal children, the level of IFN-γin blood plasma of the patients was significantly increased(P<0.05),while the level of IL-10 was significantly decreased(P<0.05). In EV71 group, the linear correlation analysis showed that linear regression equation was y=118.78-1.87x, r=-0.65,P<0.05. In CA16 group, the linear correlation analysis showed that linear regression equation was y=87.13-1.29x, r=-0.57,P<0.05.③The difference of IFN-γgene+874 site genotypes was statistically significant in EV71 group,CA16 group and control group(P<0.05); A allele frequency was higher in EV71 group and CA16 group than in normal group(P<0.05). IFN-γgene+874 site A allele frequency was higher in encephalitis group than in HFMD group(P<0.05).④The difference of IL-10 gene-1082 site genotypes was statistically significant in EV71 group,CA16 group and control group(P <0.05); A allele frequency was higher in EV71 group than in normal group(P< 0.05),while A allele frequency was no significant difference between CA16 group and control group (P>0.05). IL-10 gene-1082 site A allele frequency was higher in encephalitis group than in HFMD group(P<0.05).Conclusion:genotype of IFN-γgene+874 site, IL-10 gene-1082 site, the level of IFN-γand IL-10 in plasma might have relationship with the generation of HFMD, and A allele of IFN-γgene+874 site, A allele of IL-10 gene-1082 site might be the genetic risk factors of encephalitis occurrence.